Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 2345254..2346054 Replicon chromosome
Accession NZ_CP054232
Organism Escherichia coli strain EcPF7

Toxin (Protein)


Gene name ataT Uniprot ID F4T503
Locus tag HR071_RS11375 Protein ID WP_000342452.1
Coordinates 2345254..2345781 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4T504
Locus tag HR071_RS11380 Protein ID WP_001277107.1
Coordinates 2345788..2346054 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR071_RS11350 2340330..2341097 - 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
HR071_RS11355 2341094..2342371 - 1278 WP_000803819.1 branched chain amino acid ABC transporter permease LivM -
HR071_RS11360 2342368..2343294 - 927 WP_001295097.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
HR071_RS11365 2343342..2344451 - 1110 WP_001296485.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
HR071_RS11370 2344874..2345257 + 384 WP_000778796.1 aspartate 1-decarboxylase autocleavage activator PanM -
HR071_RS11375 2345254..2345781 - 528 WP_000342452.1 GNAT family N-acetyltransferase Toxin
HR071_RS11380 2345788..2346054 - 267 WP_001277107.1 DUF1778 domain-containing protein Antitoxin
HR071_RS11385 2346203..2347306 - 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
HR071_RS11390 2347577..2348431 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
HR071_RS11395 2348676..2349734 - 1059 WP_001042013.1 permease-like cell division protein FtsX -
HR071_RS11400 2349727..2350395 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.57 Da        Isoelectric Point: 6.6348

>T159134 WP_000342452.1 NZ_CP054232:c2345781-2345254 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Download         Length: 528 bp

>T159134 NZ_CP069963:53380-53634 [Klebsiella pneumoniae]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9929.38 Da        Isoelectric Point: 10.0863

>AT159134 WP_001277107.1 NZ_CP054232:c2346054-2345788 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT159134 NZ_CP069963:53631-53873 [Klebsiella pneumoniae]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGTAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061K5K9

Antitoxin

Source ID Structure
AlphaFold DB A0A061YQ57

References