Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1746114..1746768 Replicon chromosome
Accession NZ_CP054232
Organism Escherichia coli strain EcPF7

Toxin (Protein)


Gene name cptA Uniprot ID F4T2L4
Locus tag HR071_RS08345 Protein ID WP_000244765.1
Coordinates 1746114..1746521 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID F4T2L5
Locus tag HR071_RS08350 Protein ID WP_000354050.1
Coordinates 1746502..1746768 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR071_RS08325 1742071..1743804 - 1734 WP_000813195.1 single-stranded-DNA-specific exonuclease RecJ -
HR071_RS08330 1743810..1744520 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HR071_RS08335 1744545..1745441 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HR071_RS08340 1745553..1746074 + 522 WP_001055874.1 flavodoxin FldB -
HR071_RS08345 1746114..1746521 - 408 WP_000244765.1 protein YgfX Toxin
HR071_RS08350 1746502..1746768 - 267 WP_000354050.1 FAD assembly factor SdhE Antitoxin
HR071_RS08355 1747011..1747991 + 981 WP_000886080.1 tRNA-modifying protein YgfZ -
HR071_RS08360 1748068..1748727 - 660 WP_000250275.1 hemolysin III family protein -
HR071_RS08365 1748891..1749202 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
HR071_RS08370 1749247..1750680 + 1434 WP_001296350.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16043.95 Da        Isoelectric Point: 11.5202

>T159131 WP_000244765.1 NZ_CP054232:c1746521-1746114 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T159131 NZ_CP069962:c4800406-4800023 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10535.07 Da        Isoelectric Point: 5.1599

>AT159131 WP_000354050.1 NZ_CP054232:c1746768-1746502 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRTFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT159131 NZ_CP069962:c4800648-4800406 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A454A7D7

Antitoxin

Source ID Structure
AlphaFold DB A0A061L3F4

References