Detailed information of TA system TA159129

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 810125..810956
• Replicon: chromosome
• Accession: NZ_CP054232
• Organism: Escherichia coli strain EcPF7

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A066T988
• Locus tag: HR071_RS04155
• Protein ID: WP_000854815.1
• Coordinates: 810582..810956 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A061Y7A8
• Locus tag: HR071_RS04150
• Protein ID: WP_001280918.1
• Coordinates: 810125..810493 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR071_RS04110	805716..806393	+	678	WP_001362823.1	hypothetical protein	-
HR071_RS04115	806409..806819	+	411	WP_000846703.1	hypothetical protein	-
HR071_RS04120	807040..807858	+	819	WP_001542275.1	DUF945 domain-containing protein	-
HR071_RS04125	807858..808103	+	246	WP_001164966.1	hypothetical protein	-
HR071_RS04130	808197..808670	+	474	WP_001542276.1	antirestriction protein	-
HR071_RS04135	808686..809162	+	477	WP_001186200.1	RadC family protein	-
HR071_RS04140	809225..809446	+	222	WP_000692345.1	DUF987 domain-containing protein	-
HR071_RS04145	809465..810109	+	645	WP_000086752.1	hypothetical protein	-
HR071_RS04150	810125..810493	+	369	WP_001280918.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HR071_RS04155	810582..810956	+	375	WP_000854815.1	type IV toxin-antitoxin system toxin CbtA	Toxin
HR071_RS04160	810953..811147	+	195	WP_000988600.1	hypothetical protein	-
HR071_RS04165	811160..811273	+	114	WP_001161660.1	DUF957 domain-containing protein	-
HR071_RS04170	811562..811735	-	174	WP_032082634.1	transposase domain-containing protein	-
HR071_RS04175	811762..811944	+	183	WP_001296208.1	hypothetical protein	-
HR071_RS04180	812045..812374	-	330	WP_000450409.1	DUF496 family protein	-
HR071_RS04185	812546..813604	-	1059	WP_001200889.1	FUSC family protein	-
HR071_RS04190	813802..814275	-	474	WP_001105368.1	DNA gyrase inhibitor SbmC	-
HR071_RS04195	814394..815560	-	1167	WP_001296209.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13829.83 Da
• Isoelectric Point: 7.1326

>T159129 WP_000854815.1 NZ_CP054232:810582-810956 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTSDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T159129 NZ_CP069962:c4331857-4331573 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13811.70 Da
• Isoelectric Point: 6.4767

>AT159129 WP_001280918.1 NZ_CP054232:810125-810493 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT159129 NZ_CP069962:c4332089-4331847 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A066T988

Antitoxin

• AlphaFold DB: A0A061Y7A8