Detailed information of TA system TA159117

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4593571..4594406
• Replicon: chromosome
• Accession: NZ_CP054230
• Organism: Escherichia coli strain EcPF14

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q707F1
• Locus tag: HR072_RS22070
• Protein ID: WP_001094445.1
• Coordinates: 4594029..4594406 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HR072_RS22065
• Protein ID: WP_024174331.1
• Coordinates: 4593571..4593939 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR072_RS22035	4590327..4590782	+	456	WP_000581502.1	hypothetical protein	-
HR072_RS22040	4591064..4591885	+	822	WP_021556204.1	DUF945 domain-containing protein	-
HR072_RS22045	4591885..4592130	+	246	WP_001164966.1	hypothetical protein	-
HR072_RS22050	4592224..4592697	+	474	WP_001313575.1	antirestriction protein	-
HR072_RS22055	4592713..4593189	+	477	WP_001186193.1	RadC family protein	-
HR072_RS22060	4593276..4593497	+	222	WP_000692346.1	DUF987 domain-containing protein	-
HR072_RS22065	4593571..4593939	+	369	WP_024174331.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HR072_RS22070	4594029..4594406	+	378	WP_001094445.1	TA system toxin CbtA family protein	Toxin
HR072_RS22075	4594403..4594891	+	489	WP_001534777.1	hypothetical protein	-
HR072_RS22080	4594903..4595100	+	198	WP_000838536.1	DUF957 domain-containing protein	-
HR072_RS22085	4595185..4596027	+	843	WP_001280501.1	DUF4942 domain-containing protein	-
HR072_RS22090	4596776..4598314	+	1539	WP_001187191.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14194.10 Da
• Isoelectric Point: 6.8518

>T159117 WP_001094445.1 NZ_CP054230:4594029-4594406 [Escherichia coli]
MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRMVNNITQGKHPEAQQ

Nucleotide

• Length: 378 bp

>T159117 NZ_CP069961:2611686-2612054 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCTGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13603.42 Da
• Isoelectric Point: 6.7386

>AT159117 WP_024174331.1 NZ_CP054230:4593571-4593939 [Escherichia coli]
VSDTLPGTTHPDDNHDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKT

Nucleotide

• Length: 369 bp

>AT159117 NZ_CP069961:2611468-2611689 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A454A7U6