Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4217266..4217816 Replicon chromosome
Accession NZ_CP054230
Organism Escherichia coli strain EcPF14

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag HR072_RS20265 Protein ID WP_001160966.1
Coordinates 4217266..4217580 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag HR072_RS20270 Protein ID WP_000125566.1
Coordinates 4217583..4217816 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR072_RS20240 4212941..4214227 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
HR072_RS20245 4214227..4215216 + 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
HR072_RS20250 4215213..4216034 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
HR072_RS20255 4216037..4216414 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
HR072_RS20260 4216421..4217269 + 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
HR072_RS20265 4217266..4217580 - 315 WP_001160966.1 CcdB family protein Toxin
HR072_RS20270 4217583..4217816 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
HR072_RS20275 4217902..4218381 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
HR072_RS20280 4218573..4220435 - 1863 WP_000377123.1 glutathione-regulated potassium-efflux system protein KefC -
HR072_RS20285 4220428..4220958 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
HR072_RS20290 4221066..4222397 - 1332 WP_001183207.1 MFS transporter -
HR072_RS20295 4222456..4222743 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T159112 WP_001160966.1 NZ_CP054230:c4217580-4217266 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T159112 NZ_CP069961:c1611479-1610946 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTATTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT159112 WP_000125566.1 NZ_CP054230:c4217816-4217583 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT159112 NZ_CP069961:c1611756-1611490 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCTAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References