Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3703732..3704350 Replicon chromosome
Accession NZ_CP054230
Organism Escherichia coli strain EcPF14

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HR072_RS17860 Protein ID WP_001291435.1
Coordinates 3704132..3704350 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HR072_RS17855 Protein ID WP_000344800.1
Coordinates 3703732..3704106 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR072_RS17845 3698822..3700015 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HR072_RS17850 3700038..3703187 + 3150 WP_176316529.1 efflux RND transporter permease AcrB -
HR072_RS17855 3703732..3704106 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HR072_RS17860 3704132..3704350 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
HR072_RS17865 3704523..3705074 + 552 WP_000102539.1 maltose O-acetyltransferase -
HR072_RS17870 3705190..3705660 + 471 WP_001304825.1 YlaC family protein -
HR072_RS17875 3705824..3707374 + 1551 WP_001528761.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HR072_RS17880 3707416..3707769 - 354 WP_000878140.1 DUF1428 domain-containing protein -
HR072_RS17890 3708148..3708466 + 319 Protein_3509 MGMT family protein -
HR072_RS17895 3708497..3709069 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T159110 WP_001291435.1 NZ_CP054230:3704132-3704350 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T159110 NZ_CP069959:c79562-79413 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT159110 WP_000344800.1 NZ_CP054230:3703732-3704106 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT159110 NZ_CP069959:79606-79667 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References