Detailed information of TA system TA159090

Bioinformatically predicted

Overview

TA module

• Type: III
• Classification (family/domain): toxIN/ToxN(toxin)
• Location: 5620..6349
• Replicon: plasmid p2
• Accession: NZ_CP054229
• Organism: Escherichia coli strain EcPF15

Toxin (Protein)

• Gene name: toxN
• Uniprot ID: A0A8B6BBN5
• Locus tag: HR073_RS23275
• Protein ID: WP_000675351.1
• Coordinates: 5620..6135 (-)
• Length: 172 a.a.

Antitoxin (RNA)

Gene name	toxI
Locus tag	-
Coordinates	6137..6349 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR073_RS23255	1011..1595	+	585	Protein_0	primase C-terminal domain-containing protein	-
HR073_RS23260	1592..3271	+	1680	WP_001774712.1	ATP-binding protein	-
HR073_RS23265	3508..3702	-	195	WP_001041636.1	Rop family plasmid primer RNA-binding protein	-
HR073_RS23270	4496..5206	-	711	WP_000967645.1	hypothetical protein	-
HR073_RS23275	5620..6135	-	516	WP_000675351.1	type III toxin-antitoxin system ToxN/AbiQ family toxin	Toxin
-	6137..6349	-	213	NuclAT_0	-	Antitoxin
-	6137..6349	-	213	NuclAT_0	-	Antitoxin
-	6137..6349	-	213	NuclAT_0	-	Antitoxin
-	6137..6349	-	213	NuclAT_0	-	Antitoxin
-	6185..6277	-	93	NuclAT_5	-	-
-	6185..6277	-	93	NuclAT_5	-	-
-	6185..6277	-	93	NuclAT_5	-	-
-	6185..6277	-	93	NuclAT_5	-	-
-	6185..6313	-	129	NuclAT_3	-	-
-	6185..6313	-	129	NuclAT_3	-	-
-	6185..6313	-	129	NuclAT_3	-	-
-	6185..6313	-	129	NuclAT_3	-	-
-	6185..6350	-	166	NuclAT_1	-	-
-	6185..6350	-	166	NuclAT_1	-	-
-	6185..6350	-	166	NuclAT_1	-	-
-	6185..6350	-	166	NuclAT_1	-	-
-	6221..6349	-	129	NuclAT_2	-	-
-	6221..6349	-	129	NuclAT_2	-	-
-	6221..6349	-	129	NuclAT_2	-	-
-	6221..6349	-	129	NuclAT_2	-	-
-	6256..6349	-	94	NuclAT_4	-	-
-	6256..6349	-	94	NuclAT_4	-	-
-	6256..6349	-	94	NuclAT_4	-	-
-	6256..6349	-	94	NuclAT_4	-	-
-	6292..6349	-	58	NuclAT_6	-	-
-	6292..6349	-	58	NuclAT_6	-	-
-	6292..6349	-	58	NuclAT_6	-	-
-	6292..6349	-	58	NuclAT_6	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..6431	6431

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ToxN/AbiQ toxin (1-159)

Sequences

Toxin

Protein

• Length: 172 a.a.
• Molecular weight: 19724.66 Da
• Isoelectric Point: 6.7273

>T159090 WP_000675351.1 NZ_CP054229:c6135-5620 [Escherichia coli]
MKFYTISSRYIEYLKGFEDKVPNSEDPNYQNPKAFIGIVLEIQGHKYLAPLTSPKPWHSTIKESSPSFFKLHENGVPENQ
LGLINLKFMIPIIEAEVSLLDLNGMPNTAYKRMLYKQLQFIRVNSDKIAEKSELLRKLVLQGKMQGTCNFSLLEEKYRDF
GKEANDTEEGE

Nucleotide

• Length: 516 bp

>T159090 NZ_CP069957:1947051-1947269 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Nucleotide

• Length: 213 bp

>AT159090 NZ_CP054229:c6349-6137 [Escherichia coli]
AGGTGATTTGCTACCTTTAAGTGCAGCTAGAAATTCAGGTGATTTGCTACCTTTAAGTGCAGCTAGAAATTCAGGTGATT
TGCTACCTTTAAGTGCAGCTAGAAATTCAGGTGATTTGCTACCTTAAGTGCAGCTAGAAATTTAGGTCATTTACTACCTT
AAAGTAATACAGTAAAAGAGGCGACTACACAGTCGCCTTTTTTACGAGAAAAA

Structures

Toxin

• AlphaFold DB: A0A8B6BBN5

Antitoxin