Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 129996..130521 Replicon plasmid p1
Accession NZ_CP054228
Organism Escherichia coli strain EcPF15

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag HR073_RS23220 Protein ID WP_001159868.1
Coordinates 129996..130301 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag HR073_RS23225 Protein ID WP_000813634.1
Coordinates 130303..130521 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR073_RS23200 125773..126744 - 972 WP_000817031.1 ParB/RepB/Spo0J family plasmid partition protein -
HR073_RS23205 126744..127910 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
HR073_RS23210 128498..129142 - 645 WP_001477618.1 replication initiation protein RepE -
HR073_RS23215 129189..129995 - 807 WP_024243979.1 site-specific integrase -
HR073_RS23220 129996..130301 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
HR073_RS23225 130303..130521 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HR073_RS23230 130989..131672 + 684 WP_010891291.1 DNA-binding protein -
HR073_RS23235 131669..132289 + 621 WP_001248529.1 hypothetical protein -
HR073_RS23240 132286..133074 + 789 WP_176323726.1 DUF2971 domain-containing protein -
HR073_RS23245 133110..133850 + 741 WP_000933099.1 abortive infection family protein -
HR073_RS23250 134009..134674 - 666 WP_053265868.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - papH / papC / papD / hlyB / iroB / iroC / iroD / iroE / iroN 1..135461 135461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T159089 WP_001159868.1 NZ_CP054228:c130301-129996 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T159089 NZ_CP069957:1524253-1524639 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT159089 WP_000813634.1 NZ_CP054228:c130521-130303 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT159089 NZ_CP069957:1524629-1524958 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References