Detailed information of TA system TA159088

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 84195..84820
• Replicon: plasmid p1
• Accession: NZ_CP054228
• Organism: Escherichia coli strain EcPF15

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HR073_RS22930
• Protein ID: WP_097377987.1
• Coordinates: 84422..84820 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HR073_RS22925
• Protein ID: WP_161574083.1
• Coordinates: 84195..84422 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR073_RS22925	84195..84422	+	228	WP_161574083.1	toxin-antitoxin system antitoxin VapB	Antitoxin
HR073_RS22930	84422..84820	+	399	WP_097377987.1	tRNA(fMet)-specific endonuclease VapC	Toxin
HR073_RS22935	84829..86991	-	2163	WP_176323718.1	type IV conjugative transfer system coupling protein TraD	-
HR073_RS22940	87306..87999	-	694	Protein_74	hypothetical protein	-
HR073_RS22945	88211..88519	+	309	WP_097377989.1	heavy metal-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	papH / papC / papD / hlyB / iroB / iroC / iroD / iroE / iroN	1..135461	135461

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T159088 WP_097377987.1 NZ_CP054228:84422-84820 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T159088 NZ_CP069957:1230364-1230858 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8528.55 Da
• Isoelectric Point: 4.5358

>AT159088 WP_161574083.1 NZ_CP054228:84195-84422 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGESWDEWFDGNSVSTDFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT159088 NZ_CP069957:1230074-1230367 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAACCAGGCGCGAATCTTTAAATTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA