Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 74132..74679 Replicon plasmid p1
Accession NZ_CP054228
Organism Escherichia coli strain EcPF15

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag HR073_RS22880 Protein ID WP_000969996.1
Coordinates 74132..74413 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A2I2C2Y5
Locus tag HR073_RS22885 Protein ID WP_000079948.1
Coordinates 74410..74679 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR073_RS22840 69359..69670 - 312 WP_001259758.1 colicin V -
HR073_RS22845 69648..69884 - 237 WP_001323890.1 colicin V immunity protein -
HR073_RS22850 70051..70822 + 772 Protein_56 Gfo/Idh/MocA family oxidoreductase -
HR073_RS22855 70824..71093 + 270 WP_000379710.1 SemiSWEET transporter -
HR073_RS22860 71090..71356 + 267 WP_001017350.1 NAD(P)/FAD-dependent oxidoreductase -
HR073_RS22865 71412..71864 - 453 WP_000670963.1 N-acetyltransferase -
HR073_RS22870 72138..73351 + 1214 WP_085947772.1 IS3 family transposase -
HR073_RS22875 73358..74086 - 729 Protein_61 3'-5' exonuclease -
HR073_RS22880 74132..74413 - 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HR073_RS22885 74410..74679 - 270 WP_000079948.1 hypothetical protein Antitoxin
HR073_RS22890 75594..76451 - 858 WP_176323714.1 incFII family plasmid replication initiator RepA -
HR073_RS22895 76444..76518 - 75 WP_097378006.1 RepA leader peptide Tap -
HR073_RS22900 76752..77009 - 258 WP_000083826.1 replication regulatory protein RepA -
HR073_RS22905 77172..77330 - 159 WP_172428062.1 hypothetical protein -
HR073_RS22910 77468..78028 - 561 WP_176323715.1 fertility inhibition protein FinO -
HR073_RS22915 78083..78823 - 741 WP_176323716.1 conjugal transfer pilus acetylase TraX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - papH / papC / papD / hlyB / iroB / iroC / iroD / iroE / iroN 1..135461 135461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T159087 WP_000969996.1 NZ_CP054228:c74413-74132 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T159087 NZ_CP069957:1052770-1052877 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10251.64 Da        Isoelectric Point: 10.1734

>AT159087 WP_000079948.1 NZ_CP054228:c74679-74410 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLKRGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT159087 NZ_CP069957:c1052717-1052656 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A2I2C2Y5

References