Detailed information of TA system TA159086

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/-
• Location: 64768..65144
• Replicon: plasmid p1
• Accession: NZ_CP054228
• Organism: Escherichia coli strain EcPF15

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A075MDK3
• Locus tag: HR073_RS22820
• Protein ID: WP_000969988.1
• Coordinates: 64768..64950 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: HR073_RS22825
• Protein ID: WP_137504090.1
• Coordinates: 64947..65144 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR073_RS22805	61352..62580	+	1229	WP_176323710.1	IS3 family transposase	-
HR073_RS22810	62767..63801	-	1035	WP_001190234.1	3-deoxy-7-phosphoheptulonate synthase	-
HR073_RS22815	64361..64669	-	309	WP_176323711.1	hypothetical protein	-
HR073_RS22820	64768..64950	-	183	WP_000969988.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HR073_RS22825	64947..65144	-	198	WP_137504090.1	toxin-antitoxin system protein	Antitoxin
HR073_RS22830	65826..67100	+	1275	WP_176323712.1	HlyD family secretion protein	-
HR073_RS22835	67075..69189	+	2115	WP_176323713.1	peptidase domain-containing ABC transporter	-
HR073_RS22840	69359..69670	-	312	WP_001259758.1	colicin V	-
HR073_RS22845	69648..69884	-	237	WP_001323890.1	colicin V immunity protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	papH / papC / papD / hlyB / iroB / iroC / iroD / iroE / iroN	1..135461	135461

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (88-134)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6931.07 Da
• Isoelectric Point: 4.5349

>T159086 WP_000969988.1 NZ_CP054228:c64950-64768 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGCSASLLFSLSFISVQVHDNIITV

Nucleotide

• Length: 183 bp

>T159086 NZ_CP069957:c790337-790161 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCGTGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7657.54 Da
• Isoelectric Point: 9.0188

>AT159086 WP_137504090.1 NZ_CP054228:c65144-64947 [Escherichia coli]
MDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHNMVESSAAAWRDEMSRKVAGK

Nucleotide

• Length: 198 bp

>AT159086 NZ_CP069957:c790115-789699 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A075MDK3