Detailed information of TA system TA159070

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2106958..2107596
• Replicon: chromosome
• Accession: NZ_CP054227
• Organism: Escherichia coli strain EcPF15

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: B7N4J4
• Locus tag: HR073_RS09905
• Protein ID: WP_000813797.1
• Coordinates: 2106958..2107134 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HR073_RS09910
• Protein ID: WP_076611057.1
• Coordinates: 2107180..2107596 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR073_RS09885	2102580..2103791	-	1212	WP_001323162.1	BenE family transporter YdcO	-
HR073_RS09890	2103844..2104380	+	537	WP_000429161.1	DNA-binding transcriptional regulator SutR	-
HR073_RS09895	2104453..2106414	+	1962	WP_001309488.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HR073_RS09900	2106506..2106736	-	231	WP_000494244.1	YncJ family protein	-
HR073_RS09905	2106958..2107134	+	177	WP_000813797.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HR073_RS09910	2107180..2107596	+	417	WP_076611057.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HR073_RS09915	2107675..2109081	+	1407	WP_000760613.1	PLP-dependent aminotransferase family protein	-
HR073_RS09920	2109326..2110471	+	1146	WP_000047447.1	ABC transporter substrate-binding protein	-
HR073_RS09925	2110489..2111502	+	1014	WP_176323614.1	ABC transporter ATP-binding protein	-
HR073_RS09930	2111503..2112444	+	942	WP_001251335.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6724.76 Da
• Isoelectric Point: 11.5666

>T159070 WP_000813797.1 NZ_CP054227:2106958-2107134 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T159070 NZ_CP069946:4061603-4061887 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCAGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15251.65 Da
• Isoelectric Point: 4.5908

>AT159070 WP_076611057.1 NZ_CP054227:2107180-2107596 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT159070 NZ_CP069946:4061371-4061613 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A829J715