Detailed information of TA system TA159064

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1503258..1503963
• Replicon: chromosome
• Accession: NZ_CP054227
• Organism: Escherichia coli strain EcPF15

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: HR073_RS06940
• Protein ID: WP_000539521.1
• Coordinates: 1503577..1503963 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HR073_RS06935
• Protein ID: WP_001280945.1
• Coordinates: 1503258..1503587 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR073_RS06920	1498415..1499326	+	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-
HR073_RS06925	1499504..1501852	+	2349	WP_001322378.1	EAL domain-containing protein	-
HR073_RS06930	1501860..1503188	+	1329	WP_000086879.1	GGDEF domain-containing protein	-
HR073_RS06935	1503258..1503587	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
HR073_RS06940	1503577..1503963	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HR073_RS06945	1504189..1505514	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
HR073_RS06950	1505727..1506110	+	384	WP_000497137.1	biofilm formation regulator BssR	-
HR073_RS06955	1506221..1507336	+	1116	WP_000555033.1	aldose sugar dehydrogenase YliI	-
HR073_RS06960	1507333..1507959	-	627	WP_001314584.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T159064 WP_000539521.1 NZ_CP054227:c1503963-1503577 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T159064 NZ_CP069946:c2041030-2040614 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATTATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACCGACATCCGGGTGGCGCTGCACCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCGGGCGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTGCCTGGACTGACACTGG
AAGACTGGGTTATTTAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT159064 WP_001280945.1 NZ_CP054227:c1503587-1503258 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT159064 NZ_CP069946:c2041257-2041027 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAACGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6