Detailed information of TA system TA159053

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3589727..3590406
• Replicon: chromosome
• Accession: NZ_CP054224
• Organism: Escherichia coli strain EcPF16

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: HR074_RS17355
• Protein ID: WP_000854680.1
• Coordinates: 3590065..3590406 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EWR7
• Locus tag: HR074_RS17350
• Protein ID: WP_000070396.1
• Coordinates: 3589727..3590044 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR074_RS17305	3584757..3585458	+	702	WP_176321062.1	WYL domain-containing protein	-
HR074_RS17310	3585499..3585735	+	237	WP_001144031.1	hypothetical protein	-
HR074_RS17315	3585735..3586178	+	444	WP_000649865.1	hypothetical protein	-
HR074_RS17320	3586201..3586668	+	468	WP_032238643.1	hypothetical protein	-
HR074_RS17330	3588185..3588430	+	246	WP_176321063.1	DUF905 domain-containing protein	-
HR074_RS17335	3588528..3588986	+	459	WP_000211838.1	antirestriction protein	-
HR074_RS17340	3589002..3589478	+	477	WP_000811693.1	RadC family protein	-
HR074_RS17345	3589487..3589708	+	222	WP_000691994.1	DUF987 domain-containing protein	-
HR074_RS17350	3589727..3590044	+	318	WP_000070396.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
HR074_RS17355	3590065..3590406	+	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
HR074_RS17360	3591072..3591281	+	210	Protein_3400	transposase	-
HR074_RS17365	3591323..3592066	-	744	WP_032238641.1	AraC family transcriptional regulator	-
HR074_RS17370	3592454..3593365	-	912	WP_073527469.1	IS5 family transposase	-
HR074_RS17375	3593718..3594782	+	1065	WP_072003692.1	acyltransferase	-
HR074_RS17380	3594814..3595179	-	366	WP_176321064.1	tail fiber assembly protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	3586782..3593365	6583
	-	inside	Prophage	-	-	3583719..3603090	19371

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T159053 WP_000854680.1 NZ_CP054224:3590065-3590406 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T159053 NZ_CP069945:c2904069-2903815 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGATCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11982.62 Da
• Isoelectric Point: 7.0270

>AT159053 WP_000070396.1 NZ_CP054224:3589727-3590044 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT159053 NZ_CP069945:c2904317-2904066 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: S1FHS4

Antitoxin

• AlphaFold DB: S1EWR7