Detailed information of TA system TA159051

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3014485..3015190
• Replicon: chromosome
• Accession: NZ_CP054224
• Organism: Escherichia coli strain EcPF16

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: HR074_RS14570
• Protein ID: WP_000539521.1
• Coordinates: 3014485..3014871 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HR074_RS14575
• Protein ID: WP_001280945.1
• Coordinates: 3014861..3015190 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR074_RS14550	3010489..3011115	+	627	WP_016241992.1	glutathione S-transferase GstB	-
HR074_RS14555	3011112..3012227	-	1116	WP_000555031.1	aldose sugar dehydrogenase YliI	-
HR074_RS14560	3012338..3012721	-	384	WP_000497137.1	biofilm formation regulator BssR	-
HR074_RS14565	3012934..3014259	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
HR074_RS14570	3014485..3014871	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HR074_RS14575	3014861..3015190	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
HR074_RS14580	3015260..3016588	-	1329	WP_000086877.1	GGDEF domain-containing protein	-
HR074_RS14585	3016596..3018944	-	2349	WP_032238219.1	EAL domain-containing protein	-
HR074_RS14590	3019122..3020033	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T159051 WP_000539521.1 NZ_CP054224:3014485-3014871 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T159051 NZ_CP069945:c2666395-2666293 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT159051 WP_001280945.1 NZ_CP054224:3014861-3015190 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT159051 NZ_CP069945:2666255-2666523 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Structures

Toxin

• AlphaFold DB: A0A829GCT6