159014

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4054016..4054634 Replicon chromosome
Accession NZ_CP054219
Organism Escherichia coli strain EcPF18

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HR075_RS19300 Protein ID WP_001291435.1
Coordinates 4054016..4054234 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HR075_RS19305 Protein ID WP_000344800.1
Coordinates 4054260..4054634 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR075_RS19265 4049303..4049875 + 573 WP_000779831.1 YbaY family lipoprotein -
HR075_RS19270 4049906..4050217 - 312 WP_000409908.1 MGMT family protein -
HR075_RS19280 4050596..4050949 + 354 WP_000878135.1 DUF1428 family protein -
HR075_RS19285 4050991..4052541 - 1551 WP_001385227.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HR075_RS19290 4052705..4053175 - 471 WP_000136192.1 YlaC family protein -
HR075_RS19295 4053291..4053842 - 552 WP_000102539.1 maltose O-acetyltransferase -
HR075_RS19300 4054016..4054234 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
HR075_RS19305 4054260..4054634 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HR075_RS19310 4055180..4058329 - 3150 WP_001132478.1 multidrug efflux RND transporter permease subunit -
HR075_RS19315 4058352..4059545 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T159014 WP_001291435.1 NZ_CP054219:c4054234-4054016 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T159014 NZ_CP069939:c2774897-2774514 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTACCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT159014 WP_000344800.1 NZ_CP054219:c4054634-4054260 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT159014 NZ_CP069939:c2775139-2774897 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References