Detailed information of TA system TA159010

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 3425815..3426635
• Replicon: chromosome
• Accession: NZ_CP054219
• Organism: Escherichia coli strain EcPF18

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: B6I030
• Locus tag: HR075_RS16310
• Protein ID: WP_001054379.1
• Coordinates: 3425815..3426072 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: HR075_RS16315
• Protein ID: WP_000123957.1
• Coordinates: 3426084..3426635 (+)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR075_RS16295	3423677..3423931	-	255	WP_000788354.1	glycosyltransferase	-
HR075_RS16300	3424094..3425241	+	1148	WP_085949154.1	IS3-like element ISEc52 family transposase	-
HR075_RS16305	3425274..3425438	+	165	Protein_3181	GNAT family N-acetyltransferase	-
HR075_RS16310	3425815..3426072	+	258	WP_001054379.1	YjhX family toxin	Toxin
HR075_RS16315	3426084..3426635	+	552	WP_000123957.1	N-acetyltransferase	Antitoxin
HR075_RS16320	3426687..3427433	+	747	WP_000354249.1	class I SAM-dependent methyltransferase	-
HR075_RS16325	3427561..3427821	+	261	WP_000077645.1	hypothetical protein	-
HR075_RS16330	3428220..3429341	+	1122	WP_000010833.1	M42 family metallopeptidase	-
HR075_RS16335	3429338..3429616	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
HR075_RS16340	3429628..3430941	+	1314	WP_000460845.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3413585..3434856	21271

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (19-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9568.07 Da
• Isoelectric Point: 11.1381

>T159010 WP_001054379.1 NZ_CP054219:3425815-3426072 [Escherichia coli]
MNLSRQEQRTLHVLAKGGRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRA
QLDNR

Nucleotide

• Length: 258 bp

>T159010 NZ_CP069939:1578791-1579009 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20358.34 Da
• Isoelectric Point: 6.4182

>AT159010 WP_000123957.1 NZ_CP054219:3426084-3426635 [Escherichia coli]
MTAHHFTFQITDESDASDIREVETRAFGFSKEADLTASLLEDESARPALSLLARHEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQALTA
QPMNVTGHIQCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT159010 NZ_CP069939:1578390-1578764 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: B6I030