Detailed information of TA system TA159002

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1883258..1884092
• Replicon: chromosome
• Accession: NZ_CP054219
• Organism: Escherichia coli strain EcPF18

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PLF5
• Locus tag: HR075_RS08980
• Protein ID: WP_000854690.1
• Coordinates: 1883715..1884092 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1P7N8
• Locus tag: HR075_RS08975
• Protein ID: WP_001305076.1
• Coordinates: 1883258..1883626 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR075_RS08935	1878340..1879467	+	1128	Protein_1752	hypothetical protein	-
HR075_RS08940	1879543..1879998	+	456	WP_000581502.1	hypothetical protein	-
HR075_RS08945	1880077..1880310	+	234	WP_000902034.1	DUF905 family protein	-
HR075_RS08950	1880411..1881229	+	819	WP_001234620.1	DUF945 domain-containing protein	-
HR075_RS08955	1881284..1881769	+	486	WP_000849565.1	antirestriction protein	-
HR075_RS08960	1881785..1882261	+	477	WP_001186726.1	RadC family protein	-
HR075_RS08965	1882324..1882545	+	222	WP_000692329.1	DUF987 domain-containing protein	-
HR075_RS08970	1882564..1883208	+	645	WP_000094916.1	hypothetical protein	-
HR075_RS08975	1883258..1883626	+	369	WP_001305076.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HR075_RS08980	1883715..1884092	+	378	WP_000854690.1	TA system toxin CbtA family protein	Toxin
HR075_RS08985	1884089..1884577	+	489	WP_000761699.1	hypothetical protein	-
HR075_RS08990	1884594..1884791	+	198	WP_000839293.1	DUF957 domain-containing protein	-
HR075_RS08995	1884876..1885721	+	846	WP_001529401.1	DUF4942 domain-containing protein	-
HR075_RS09000	1885790..1886185	+	396	WP_000208384.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
HR075_RS09005	1886178..1887111	+	934	Protein_1766	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
HR075_RS09010	1887543..1887698	+	156	WP_000729638.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1887543..1887698	155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14057.04 Da
• Isoelectric Point: 9.1510

>T159002 WP_000854690.1 NZ_CP054219:1883715-1884092 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYAQVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T159002 NZ_CP069935:3938506-3938817 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13560.39 Da
• Isoelectric Point: 4.7830

>AT159002 WP_001305076.1 NZ_CP054219:1883258-1883626 [Escherichia coli]
MSDTLPGTTLPDDNKDLPWWGLPCTVTPCFGACLVQEGNRLHYLADRAGIRGRFSDADAYHPDQAFPLLMKQPELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT159002 NZ_CP069935:3938818-3939108 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1PLF5

Antitoxin

• AlphaFold DB: S1P7N8