Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1738345..1738999 Replicon chromosome
Accession NZ_CP054219
Organism Escherichia coli strain EcPF18

Toxin (Protein)


Gene name cptA Uniprot ID F4T2L4
Locus tag HR075_RS08225 Protein ID WP_000244765.1
Coordinates 1738345..1738752 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID F4T2L5
Locus tag HR075_RS08230 Protein ID WP_000354050.1
Coordinates 1738733..1738999 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR075_RS08205 1734302..1736035 - 1734 WP_000813195.1 single-stranded-DNA-specific exonuclease RecJ -
HR075_RS08210 1736041..1736751 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HR075_RS08215 1736776..1737672 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HR075_RS08220 1737784..1738305 + 522 WP_001055874.1 flavodoxin FldB -
HR075_RS08225 1738345..1738752 - 408 WP_000244765.1 protein YgfX Toxin
HR075_RS08230 1738733..1738999 - 267 WP_000354050.1 FAD assembly factor SdhE Antitoxin
HR075_RS08235 1739242..1740222 + 981 WP_000886084.1 tRNA-modifying protein YgfZ -
HR075_RS08240 1740299..1740958 - 660 WP_000250275.1 hemolysin III family protein -
HR075_RS08245 1741122..1741433 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
HR075_RS08250 1741478..1742911 + 1434 WP_001296350.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16043.95 Da        Isoelectric Point: 11.5202

>T159001 WP_000244765.1 NZ_CP054219:c1738752-1738345 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T159001 NZ_CP069935:3667135-3667512 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCATCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10535.07 Da        Isoelectric Point: 5.1599

>AT159001 WP_000354050.1 NZ_CP054219:c1738999-1738733 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRTFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT159001 NZ_CP069935:3666711-3667088 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCATCACACTGTATGCGAAGGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGCTTATCTGGCTGTTTATCCGACACCGGCAGAGCCCGCAATCACCGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A454A7D7

Antitoxin

Source ID Structure
AlphaFold DB A0A061L3F4

References