Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 805056..805887 Replicon chromosome
Accession NZ_CP054219
Organism Escherichia coli strain EcPF18

Toxin (Protein)


Gene name yeeV Uniprot ID A0A066T988
Locus tag HR075_RS04045 Protein ID WP_000854815.1
Coordinates 805513..805887 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A061Y7A8
Locus tag HR075_RS04040 Protein ID WP_001280918.1
Coordinates 805056..805424 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR075_RS03995 800145..800891 + 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -
HR075_RS04000 800974..801324 + 351 Protein_788 hypothetical protein -
HR075_RS04005 801340..801750 + 411 WP_000846703.1 hypothetical protein -
HR075_RS04010 801971..802789 + 819 WP_001542275.1 DUF945 domain-containing protein -
HR075_RS04015 802789..803034 + 246 WP_001164966.1 hypothetical protein -
HR075_RS04020 803128..803601 + 474 WP_001542276.1 antirestriction protein -
HR075_RS04025 803617..804093 + 477 WP_001186200.1 RadC family protein -
HR075_RS04030 804156..804377 + 222 WP_000692345.1 DUF987 domain-containing protein -
HR075_RS04035 804396..805040 + 645 WP_000086752.1 hypothetical protein -
HR075_RS04040 805056..805424 + 369 WP_001280918.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HR075_RS04045 805513..805887 + 375 WP_000854815.1 type IV toxin-antitoxin system toxin CbtA Toxin
HR075_RS04050 805884..806078 + 195 WP_000988600.1 hypothetical protein -
HR075_RS04055 806091..806204 + 114 WP_001161660.1 DUF957 domain-containing protein -
HR075_RS04060 806493..806639 - 147 Protein_800 transposase domain-containing protein -
HR075_RS04065 806693..806875 + 183 WP_001296208.1 hypothetical protein -
HR075_RS04070 806976..807305 - 330 WP_000450409.1 DUF496 family protein -
HR075_RS04075 807477..808535 - 1059 WP_001200889.1 FUSC family protein -
HR075_RS04080 808733..809206 - 474 WP_001105368.1 DNA gyrase inhibitor SbmC -
HR075_RS04085 809325..810491 - 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13829.83 Da        Isoelectric Point: 7.1326

>T158999 WP_000854815.1 NZ_CP054219:805513-805887 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTSDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T158999 NZ_CP069935:c3505363-3505256 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCACGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13811.70 Da        Isoelectric Point: 6.4767

>AT158999 WP_001280918.1 NZ_CP054219:805056-805424 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT158999 NZ_CP069935:3505412-3505477 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A066T988

Antitoxin

Source ID Structure
AlphaFold DB A0A061Y7A8

References