Detailed information of TA system TA158985

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 1940..2499
• Replicon: plasmid p2
• Accession: NZ_CP054216
• Organism: Escherichia coli strain EcPF40

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HR076_RS24840
• Protein ID: WP_000421256.1
• Coordinates: 2224..2499 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A5I3EQ05
• Locus tag: HR076_RS24835
• Protein ID: WP_001674495.1
• Coordinates: 1940..2224 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR076_RS24830	1..1032	+	1032	WP_000907863.1	replication initiation protein	-
HR076_RS24835	1940..2224	+	285	WP_001674495.1	ribbon-helix-helix domain-containing protein	Antitoxin
HR076_RS24840	2224..2499	+	276	WP_000421256.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HR076_RS24845	2605..2886	+	282	WP_001105066.1	hypothetical protein	-
HR076_RS24850	3078..3344	-	267	WP_000627831.1	hypothetical protein	-
HR076_RS24855	3344..3850	-	507	WP_042095155.1	hypothetical protein	-
HR076_RS24860	3906..4508	-	603	WP_023143808.1	hypothetical protein	-
HR076_RS24865	4862..6208	+	1347	WP_042078371.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..88657	88657

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 9.9324

>T158985 WP_000421256.1 NZ_CP054216:2224-2499 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T158985 NZ_CP069912:126632-126937 [Klebsiella oxytoca]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10460.87 Da
• Isoelectric Point: 5.6558

>AT158985 WP_001674495.1 NZ_CP054216:1940-2224 [Escherichia coli]
MQMKNNSAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT158985 NZ_CP069912:126412-126630 [Klebsiella oxytoca]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Structures

Antitoxin

• AlphaFold DB: A0A5I3EQ05