Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 72660..73185 Replicon plasmid p1
Accession NZ_CP054215
Organism Escherichia coli strain EcPF40

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag HR076_RS24740 Protein ID WP_001159868.1
Coordinates 72660..72965 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag HR076_RS24745 Protein ID WP_000813634.1
Coordinates 72967..73185 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR076_RS24725 68570..69736 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
HR076_RS24730 70324..71079 - 756 WP_000852146.1 replication initiation protein RepE -
HR076_RS24735 71853..72659 - 807 WP_000016982.1 site-specific integrase -
HR076_RS24740 72660..72965 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
HR076_RS24745 72967..73185 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HR076_RS24750 73752..74264 + 513 WP_000151784.1 hypothetical protein -
HR076_RS24755 74298..75431 - 1134 WP_001542067.1 DUF3800 domain-containing protein -
HR076_RS24760 75598..76371 - 774 WP_000905949.1 hypothetical protein -
HR076_RS24765 76384..76884 - 501 WP_001773886.1 hypothetical protein -
HR076_RS24770 77149..77379 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
HR076_RS24775 77376..77792 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / tet(B) - 1..89178 89178


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T158982 WP_001159868.1 NZ_CP054215:c72965-72660 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T158982 NZ_CP069907:5197989-5198306 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGTCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT158982 WP_000813634.1 NZ_CP054215:c73185-72967 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT158982 NZ_CP069907:5198299-5198586 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References