Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 728012..728562 Replicon chromosome
Accession NZ_CP054214
Organism Escherichia coli strain EcPF40

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag HR076_RS03485 Protein ID WP_001160966.1
Coordinates 728248..728562 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag HR076_RS03480 Protein ID WP_000125568.1
Coordinates 728012..728245 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR076_RS03455 723085..723372 + 288 WP_000203741.1 ferredoxin-like protein FixX -
HR076_RS03460 723431..724762 + 1332 WP_001183207.1 MFS transporter -
HR076_RS03465 724870..725400 + 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
HR076_RS03470 725393..727255 + 1863 WP_000377109.1 glutathione-regulated potassium-efflux system protein KefC -
HR076_RS03475 727447..727926 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
HR076_RS03480 728012..728245 + 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
HR076_RS03485 728248..728562 + 315 WP_001160966.1 CcdB family protein Toxin
HR076_RS03490 728559..729407 - 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
HR076_RS03495 729414..729791 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
HR076_RS03500 729794..730615 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
HR076_RS03505 730612..731601 - 990 WP_000241225.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
HR076_RS03510 731601..732887 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T158963 WP_001160966.1 NZ_CP054214:728248-728562 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T158963 NZ_CP069903:85557-85862 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCTGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT158963 WP_000125568.1 NZ_CP054214:728012-728245 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT158963 NZ_CP069903:85337-85555 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGAATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References