Detailed information of TA system TA158953

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 397078..397643
• Replicon: plasmid unnamed1
• Accession: NZ_CP054208
• Organism: Rhodococcus qingshengii strain CX-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: C3JNM9
• Locus tag: HRR33_RS32415
• Protein ID: WP_003943396.1
• Coordinates: 397287..397643 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: HRR33_RS32410
• Protein ID: WP_209448893.1
• Coordinates: 397078..397290 (+)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRR33_RS32380	393004..393162	+	159	WP_209448890.1	hypothetical protein	-
HRR33_RS32385	393355..393648	+	294	WP_209448891.1	hypothetical protein	-
HRR33_RS32390	394467..394712	-	246	WP_209448892.1	hypothetical protein	-
HRR33_RS32395	395237..395335	-	99	Protein_388	RNA polymerase sigma factor SigF	-
HRR33_RS35605	395312..395893	+	582	WP_257233062.1	hypothetical protein	-
HRR33_RS32405	396423..396671	+	249	WP_054828509.1	hypothetical protein	-
HRR33_RS32410	397078..397290	+	213	WP_209448893.1	ribbon-helix-helix domain-containing protein	Antitoxin
HRR33_RS32415	397287..397643	+	357	WP_003943396.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HRR33_RS32420	397841..398230	-	390	WP_209448894.1	helix-turn-helix transcriptional regulator	-
HRR33_RS32425	398769..399026	+	258	WP_209448791.1	transposase	-
HRR33_RS32430	399129..399368	-	240	WP_209448895.1	hypothetical protein	-
HRR33_RS32435	399471..400190	+	720	WP_257233083.1	DDE-type integrase/transposase/recombinase	-
HRR33_RS32440	400587..401678	-	1092	WP_209448897.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..523504	523504

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-117)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-39)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12844.58 Da
• Isoelectric Point: 6.8817

>T158953 WP_003943396.1 NZ_CP054208:397287-397643 [Rhodococcus qingshengii]
MIRRAHIHWVDLDPAQGSEAAKRRPAVVVSNDRANATAENLGRGVITVVPLTTNTAHVFPFQVIVESDESGLARASKAQA
EQVRSVSVRRLDPEPVGKVGMETFAALEDALRLHLDLQ

Nucleotide

• Length: 357 bp

>T158953 NZ_CP069893:c4511085-4510591 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGATCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 7668.40 Da
• Isoelectric Point: 4.1824

>AT158953 WP_209448893.1 NZ_CP054208:397078-397290 [Rhodococcus qingshengii]
MTTKFTISIPDEDAHLLDEYVRDHGLRGRSAGIRAALRLLSTSGLERDYAAAFGEGDVDEVWDAVTADTE

Nucleotide

• Length: 213 bp

>AT158953 NZ_CP069893:c4511375-4511082 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAGCCAGGCGCGAATCTTTAAACTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTATCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Structures

Toxin

• AlphaFold DB: A0A1F2PUJ5