Detailed information of TA system TA158950

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 897697..898290
• Replicon: chromosome
• Accession: NZ_CP054205
• Organism: Pseudomonas rhodesiae strain NL2019

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A2G0W982
• Locus tag: HRH33_RS03855
• Protein ID: WP_040265967.1
• Coordinates: 897988..898290 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HRH33_RS03850
• Protein ID: WP_167416819.1
• Coordinates: 897697..897984 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRH33_RS03830	894094..896154	-	2061	WP_167416816.1	TonB-dependent copper receptor	-
HRH33_RS03835	896249..896650	-	402	WP_167416817.1	DUF2946 domain-containing protein	-
HRH33_RS03840	896662..897156	-	495	WP_167416818.1	copper chaperone PCu(A)C	-
HRH33_RS03845	897204..897599	-	396	WP_123479525.1	DUF2946 domain-containing protein	-
HRH33_RS03850	897697..897984	-	288	WP_167416819.1	putative addiction module antidote protein	Antitoxin
HRH33_RS03855	897988..898290	-	303	WP_040265967.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HRH33_RS03860	898775..899509	-	735	WP_167416820.1	cobalt-precorrin-6A reductase	-
HRH33_RS03865	899506..900711	-	1206	WP_167416821.1	precorrin-6y C5,15-methyltransferase (decarboxylating) subunit CbiE	-
HRH33_RS03870	900834..902096	+	1263	WP_167416834.1	precorrin-3B synthase	-
HRH33_RS03875	902089..902715	+	627	WP_034095672.1	precorrin-8X methylmutase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-96)

Antitoxin

• Uncharacterized protein HI1420 (4-93)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11709.40 Da
• Isoelectric Point: 10.1888

>T158950 WP_040265967.1 NZ_CP054205:c898290-897988 [Pseudomonas rhodesiae]
MIQFKKSKHFQEWLDSLRSKPARARVLARLDNAQWGNFGDCEALGNGVFEMRIHYGPGYRVYFTRRGEVVYLLLIGGDKS
TQKRDIQRAKQIAEDFEKKE

Nucleotide

• Length: 303 bp

>T158950 NZ_CP069893:c3823621-3823403 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10333.75 Da
• Isoelectric Point: 4.4791

>AT158950 WP_167416819.1 NZ_CP054205:c897984-897697 [Pseudomonas rhodesiae]
MNEFTDFDVAEHLRTPEDMAEYLEACFEEDAGDGVLIRSALNNIARAQGMTQVARDAGLGRESLYKALSSGGNPEFATIM
KVMKALGLKLHATVI

Nucleotide

• Length: 288 bp

>AT158950 NZ_CP069893:c3824021-3823647 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A2G0W982