Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | hipBA/HipA-HipB |
| Location | 84083..85706 | Replicon | plasmid pHNTH9F11-1 |
| Accession | NZ_CP054192 | ||
| Organism | Escherichia coli strain TH9F11 | ||
Toxin (Protein)
| Gene name | hipA | Uniprot ID | H9TH67 |
| Locus tag | HRL54_RS24045 | Protein ID | WP_000088046.1 |
| Coordinates | 84381..85706 (+) | Length | 442 a.a. |
Antitoxin (Protein)
| Gene name | hipB | Uniprot ID | A0A5Z6V2D1 |
| Locus tag | HRL54_RS24040 | Protein ID | WP_024136327.1 |
| Coordinates | 84083..84376 (+) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HRL54_RS24015 (79955) | 79955..81196 | + | 1242 | WP_001053338.1 | VWA domain-containing protein | - |
| HRL54_RS26370 (81287) | 81287..81418 | + | 132 | WP_000374059.1 | hypothetical protein | - |
| HRL54_RS24020 (81759) | 81759..82040 | - | 282 | WP_000077926.1 | hypothetical protein | - |
| HRL54_RS24025 (82090) | 82090..82281 | - | 192 | WP_000398480.1 | hypothetical protein | - |
| HRL54_RS24030 (82373) | 82373..82714 | - | 342 | WP_001151575.1 | hypothetical protein | - |
| HRL54_RS24035 (83087) | 83087..83479 | + | 393 | WP_000341066.1 | hypothetical protein | - |
| HRL54_RS24040 (84083) | 84083..84376 | + | 294 | WP_024136327.1 | helix-turn-helix transcriptional regulator | Antitoxin |
| HRL54_RS24045 (84381) | 84381..85706 | + | 1326 | WP_000088046.1 | type II toxin-antitoxin system HipA family toxin | Toxin |
| HRL54_RS24050 (85767) | 85767..85973 | + | 207 | WP_000134171.1 | hypothetical protein | - |
| HRL54_RS24055 (86075) | 86075..86485 | + | 411 | WP_000985911.1 | hypothetical protein | - |
| HRL54_RS24060 (86498) | 86498..86743 | + | 246 | Protein_84 | HNH endonuclease | - |
| HRL54_RS24065 (86796) | 86796..87500 | - | 705 | WP_001067858.1 | IS6-like element IS26 family transposase | - |
| HRL54_RS24070 (87547) | 87547..88122 | - | 576 | WP_072740834.1 | recombinase family protein | - |
| HRL54_RS24075 (88248) | 88248..88598 | - | 351 | WP_000454193.1 | DUF3330 domain-containing protein | - |
| HRL54_RS24080 (88801) | 88801..89814 | - | 1014 | WP_000845048.1 | class 1 integron integrase IntI1 | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aac(3)-IVa / aph(4)-Ia / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / blaNDM-5 / tet(A) / blaCTX-M-55 / fosA3 / dfrA1 / sitABCD / qnrS1 / ARR-3 / blaOXA-10 / dfrA14 / floR | iroB / iroC / iroD / iroE / iroN | 1..407456 | 407456 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 442 a.a. Molecular weight: 50249.20 Da Isoelectric Point: 6.1927
>T158934 WP_000088046.1 NZ_CP054192:84381-85706 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK
Download Length: 1326 bp
>T158934 NZ_CP069890:c4883174-4882923 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA
Antitoxin
Download Length: 98 a.a. Molecular weight: 10946.35 Da Isoelectric Point: 10.0925
>AT158934 WP_024136327.1 NZ_CP054192:84083-84376 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW
Download Length: 294 bp
>AT158934 NZ_CP069890:c4882926-4882672 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3T0VBU1 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A5Z6V2D1 |