Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4239389..4239984 Replicon chromosome
Accession NZ_CP054191
Organism Escherichia coli strain TH9F11

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag HRL54_RS20645 Protein ID WP_000239579.1
Coordinates 4239389..4239739 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag HRL54_RS20650 Protein ID WP_001223208.1
Coordinates 4239733..4239984 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRL54_RS20625 (4234665) 4234665..4235687 - 1023 WP_001296689.1 ABC transporter permease -
HRL54_RS20630 (4235701) 4235701..4237203 - 1503 WP_000205794.1 sugar ABC transporter ATP-binding protein -
HRL54_RS20635 (4237513) 4237513..4238469 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
HRL54_RS20640 (4238779) 4238779..4239309 + 531 WP_000055072.1 inorganic diphosphatase -
HRL54_RS20645 (4239389) 4239389..4239739 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
HRL54_RS20650 (4239733) 4239733..4239984 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
HRL54_RS20655 (4240196) 4240196..4240537 - 342 WP_235153893.1 gamma-glutamylcyclotransferase -
HRL54_RS20660 (4240540) 4240540..4244319 - 3780 WP_000060895.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T158931 WP_000239579.1 NZ_CP054191:c4239739-4239389 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T158931 NZ_CP069890:c4604157-4604055 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT158931 WP_001223208.1 NZ_CP054191:c4239984-4239733 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT158931 NZ_CP069890:4604017-4604285 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References