Detailed information of TA system TA158929

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3542583..3543420
• Replicon: chromosome
• Accession: NZ_CP054191
• Organism: Escherichia coli strain TH9F11

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: HRL54_RS17215
• Protein ID: WP_000227784.1
• Coordinates: 3542878..3543420 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: HRL54_RS17210
• Protein ID: WP_001297137.1
• Coordinates: 3542583..3542894 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRL54_RS17185 (3537603)	3537603..3538550	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
HRL54_RS17190 (3538572)	3538572..3540563	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
HRL54_RS17195 (3540553)	3540553..3541167	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
HRL54_RS17200 (3541167)	3541167..3541496	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
HRL54_RS17205 (3541508)	3541508..3542398	+	891	WP_000971336.1	heme o synthase	-
HRL54_RS17210 (3542583)	3542583..3542894	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
HRL54_RS17215 (3542878)	3542878..3543420	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
HRL54_RS17220 (3543476)	3543476..3544402	-	927	WP_077890888.1	tetratricopeptide repeat protein	-
HRL54_RS17225 (3544810)	3544810..3546174	+	1365	WP_001000974.1	MFS transporter	-
HRL54_RS17230 (3546302)	3546302..3546793	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
HRL54_RS17235 (3546961)	3546961..3547872	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T158929 WP_000227784.1 NZ_CP054191:3542878-3543420 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T158929 NZ_CP069890:c4264229-4263951 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT158929 WP_001297137.1 NZ_CP054191:3542583-3542894 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT158929 NZ_CP069890:c4263951-4263667 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCGGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3