Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2485778..2486416 Replicon chromosome
Accession NZ_CP054191
Organism Escherichia coli strain TH9F11

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag HRL54_RS12035 Protein ID WP_000813794.1
Coordinates 2486240..2486416 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag HRL54_RS12030 Protein ID WP_001270286.1
Coordinates 2485778..2486194 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRL54_RS12010 (2480930) 2480930..2481871 - 942 WP_001251315.1 ABC transporter permease -
HRL54_RS12015 (2481872) 2481872..2482885 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
HRL54_RS12020 (2482903) 2482903..2484048 - 1146 WP_072644797.1 ABC transporter substrate-binding protein -
HRL54_RS12025 (2484293) 2484293..2485699 - 1407 WP_000760592.1 PLP-dependent aminotransferase family protein -
HRL54_RS12030 (2485778) 2485778..2486194 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
HRL54_RS12035 (2486240) 2486240..2486416 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
HRL54_RS12040 (2486638) 2486638..2486868 + 231 WP_000494244.1 YncJ family protein -
HRL54_RS12045 (2486960) 2486960..2488921 - 1962 WP_001593323.1 23S rRNA 5-hydroxycytidine C2501 synthase -
HRL54_RS12050 (2488994) 2488994..2489530 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
HRL54_RS12055 (2489622) 2489622..2490797 + 1176 WP_001236215.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2490837..2491985 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T158927 WP_000813794.1 NZ_CP054191:c2486416-2486240 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T158927 NZ_CP069890:3785020-3785394 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGTATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATAACCCTGGGTAAGCATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT158927 WP_001270286.1 NZ_CP054191:c2486194-2485778 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT158927 NZ_CP069890:3784596-3784973 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCACCGGTATCAGAGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGAAGCTGATACCCTTGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCACCCGCAATCACCGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References