Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 912115..912769 Replicon chromosome
Accession NZ_CP054191
Organism Escherichia coli strain TH9F11

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag HRL54_RS04485 Protein ID WP_000244772.1
Coordinates 912362..912769 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HRL54_RS04480 Protein ID WP_000354046.1
Coordinates 912115..912381 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRL54_RS04455 (907403) 907403..908146 + 744 WP_000951964.1 SDR family oxidoreductase -
HRL54_RS04460 (908203) 908203..909636 - 1434 WP_024198262.1 6-phospho-beta-glucosidase BglA -
HRL54_RS04465 (909681) 909681..909992 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
HRL54_RS04470 (910156) 910156..910815 + 660 WP_000250274.1 hemolysin III family protein -
HRL54_RS04475 (910892) 910892..911872 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
HRL54_RS04480 (912115) 912115..912381 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HRL54_RS04485 (912362) 912362..912769 + 408 WP_000244772.1 protein YgfX Toxin
HRL54_RS04490 (912809) 912809..913330 - 522 WP_001055874.1 flavodoxin FldB -
HRL54_RS04495 (913442) 913442..914338 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HRL54_RS04500 (914363) 914363..915073 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HRL54_RS04505 (915079) 915079..916812 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T158913 WP_000244772.1 NZ_CP054191:912362-912769 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T158913 NZ_CP069885:62382-62762 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACGGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGCCACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT158913 WP_000354046.1 NZ_CP054191:912115-912381 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT158913 NZ_CP069885:62161-62382 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References