Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 265365..265996 Replicon chromosome
Accession NZ_CP054191
Organism Escherichia coli strain TH9F11

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag HRL54_RS01195 Protein ID WP_001259388.1
Coordinates 265365..265640 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag HRL54_RS01200 Protein ID WP_000593555.1
Coordinates 265637..265996 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRL54_RS01180 (260369) 260369..261436 + 1068 WP_000361485.1 HlyD family secretion protein -
HRL54_RS01185 (261433) 261433..264168 + 2736 WP_000149125.1 ribosome-associated ATPase/putative transporter RbbA -
HRL54_RS01190 (264168) 264168..265292 + 1125 WP_001314210.1 ABC transporter permease -
HRL54_RS01195 (265365) 265365..265640 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
HRL54_RS01200 (265637) 265637..265996 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HRL54_RS01205 (266116) 266116..266517 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
HRL54_RS01210 (266523) 266523..267329 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
HRL54_RS01215 (267326) 267326..268090 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
HRL54_RS01220 (268090) 268090..268923 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
HRL54_RS01225 (268920) 268920..269864 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T158910 WP_001259388.1 NZ_CP054191:265365-265640 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T158910 NZ_CP069883:76029-76163 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT158910 WP_000593555.1 NZ_CP054191:265637-265996 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT158910 NZ_CP069883:c75984-75924 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References