Detailed information of TA system TA158909

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1329874..1330790
• Replicon: chromosome
• Accession: NZ_CP054177
• Organism: Bacillus subtilis strain JCL16

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: HR084_RS06940
• Protein ID: WP_089172519.1
• Coordinates: 1330044..1330790 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: G4EXD8
• Locus tag: HR084_RS06935
• Protein ID: WP_003232646.1
• Coordinates: 1329874..1330044 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR084_RS06900	1326740..1327066	+	327	WP_173614041.1	XkdW family protein	-
HR084_RS06905	1327063..1327227	+	165	WP_134155238.1	XkdX family protein	-
HR084_RS06910	1327271..1328110	+	840	WP_173614042.1	phage-like element PBSX protein XepA	-
HR084_RS06915	1328163..1328432	+	270	WP_003232655.1	hemolysin XhlA family protein	-
HR084_RS06920	1328445..1328708	+	264	WP_173614043.1	phage holin	-
HR084_RS06925	1328721..1329614	+	894	WP_029317679.1	N-acetylmuramoyl-L-alanine amidase	-
HR084_RS06930	1329651..1329788	-	138	WP_003232648.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISC	-
HR084_RS06935	1329874..1330044	-	171	WP_003232646.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISB	Antitoxin
HR084_RS06940	1330044..1330790	-	747	WP_089172519.1	toxin-antitoxin-antitoxin system toxin SpoIISA	Toxin
HR084_RS06945	1330900..1331901	-	1002	WP_003232642.1	inorganic phosphate transporter	-
HR084_RS06950	1331914..1332531	-	618	WP_003218470.1	DUF47 domain-containing protein	-
HR084_RS06955	1332807..1334123	-	1317	WP_029317680.1	serine/threonine exchanger	-
HR084_RS06960	1334512..1335462	+	951	WP_014476561.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1294496..1340682	46186

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29121.60 Da
• Isoelectric Point: 4.6191

>T158909 WP_089172519.1 NZ_CP054177:c1330790-1330044 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMFAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Nucleotide

• Length: 747 bp

>T158909 NZ_CP069883:11513-11818 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6654.72 Da
• Isoelectric Point: 11.0203

>AT158909 WP_003232646.1 NZ_CP054177:c1330044-1329874 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Nucleotide

• Length: 171 bp

>AT158909 NZ_CP069883:11293-11511 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Structures

Antitoxin

• PDB: 3O6Q
• AlphaFold DB: G4EXD8