Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 522704..523340 Replicon chromosome
Accession NZ_CP054177
Organism Bacillus subtilis strain JCL16

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag HR084_RS02685 Protein ID WP_003156187.1
Coordinates 522990..523340 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag HR084_RS02680 Protein ID WP_003225183.1
Coordinates 522704..522985 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR084_RS02660 519064..519663 - 600 WP_029317326.1 rhomboid family intramembrane serine protease -
HR084_RS02665 519758..520123 + 366 WP_015252768.1 holo-ACP synthase -
HR084_RS02670 520289..521305 + 1017 WP_173614400.1 outer membrane lipoprotein carrier protein LolA -
HR084_RS02675 521419..522588 + 1170 WP_173613923.1 alanine racemase -
HR084_RS02680 522704..522985 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
HR084_RS02685 522990..523340 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
HR084_RS02690 523455..524279 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
HR084_RS02695 524284..524649 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
HR084_RS02700 524653..525054 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
HR084_RS02705 525066..526073 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
HR084_RS02710 526135..526464 + 330 WP_080528943.1 anti-sigma factor antagonist RsbV -
HR084_RS02715 526461..526943 + 483 WP_003234299.1 anti-sigma B factor RsbW -
HR084_RS02720 526909..527697 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
HR084_RS02725 527697..528296 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T158908 WP_003156187.1 NZ_CP054177:522990-523340 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T158908 NZ_CP069883:c4395-3979 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT158908 WP_003225183.1 NZ_CP054177:522704-522985 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT158908 NZ_CP069883:c4622-4392 [Escherichia coli]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References