Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 17459..18102 Replicon plasmid pIncFIBpQil
Accession NZ_CP054172
Organism Klebsiella pneumoniae strain BA12818

Toxin (Protein)


Gene name vagD Uniprot ID A0A5Q9LNG2
Locus tag HBJ12_RS26585 Protein ID WP_048983057.1
Coordinates 17686..18102 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag HBJ12_RS26580 Protein ID WP_001261276.1
Coordinates 17459..17689 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HBJ12_RS26550 12647..13120 + 474 WP_004152341.1 YkgJ family cysteine cluster protein -
HBJ12_RS26555 14334..15116 - 783 WP_004152340.1 site-specific integrase -
HBJ12_RS26560 15116..15448 - 333 WP_004152339.1 hypothetical protein -
HBJ12_RS26565 15455..15853 - 399 WP_004171440.1 hypothetical protein -
HBJ12_RS26570 15879..16208 - 330 WP_004152337.1 hypothetical protein -
HBJ12_RS26575 16236..16544 - 309 WP_004152336.1 hypothetical protein -
HBJ12_RS26580 17459..17689 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HBJ12_RS26585 17686..18102 + 417 WP_048983057.1 type II toxin-antitoxin system VapC family toxin Toxin
HBJ12_RS26590 18278..18835 + 558 WP_193333061.1 hypothetical protein -
HBJ12_RS26595 18869..19566 + 698 Protein_18 IS1 family transposase -
HBJ12_RS26600 19789..20493 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
HBJ12_RS26605 20797..21672 + 876 WP_000239590.1 class A extended-spectrum beta-lactamase CTX-M-15 -
HBJ12_RS26610 21719..22051 - 333 WP_001393253.1 WbuC family cupin fold metalloprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-15 / qacE / dfrA30 - 1..32976 32976
- flank IS/Tn - - 11259..12527 1268


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15022.52 Da        Isoelectric Point: 9.2957

>T158907 WP_048983057.1 NZ_CP054172:17686-18102 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFARVPGLVLEDWVK

Download         Length: 417 bp

>T158907 NZ_CP069882:c5172133-5171726 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGAAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGTGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT158907 WP_001261276.1 NZ_CP054172:17459-17689 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT158907 NZ_CP069882:c5172380-5172114 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAGTGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LNG2

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References