Detailed information of TA system TA158899

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 4644299..4645110
• Replicon: chromosome
• Accession: NZ_CP054170
• Organism: Klebsiella pneumoniae strain BA12818

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: HBJ12_RS22800
• Protein ID: WP_193333055.1
• Coordinates: 4644299..4644835 (-)
• Length: 179 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: -
• Locus tag: HBJ12_RS22805
• Protein ID: WP_151361316.1
• Coordinates: 4644841..4645110 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HBJ12_RS22795	4643132..4644252	+	1121	Protein_4467	cupin domain-containing protein	-
HBJ12_RS22800	4644299..4644835	-	537	WP_193333055.1	GNAT family N-acetyltransferase	Toxin
HBJ12_RS22805	4644841..4645110	-	270	WP_151361316.1	DUF1778 domain-containing protein	Antitoxin
HBJ12_RS22810	4645213..4646644	-	1432	Protein_4470	Cu(+)/Ag(+) sensor histidine kinase	-
HBJ12_RS22815	4646634..4647316	-	683	Protein_4471	response regulator transcription factor CusR	-
HBJ12_RS22820	4647488..4648872	+	1385	Protein_4472	Cu(I)/Ag(I) efflux RND transporter outer membrane protein	-
HBJ12_RS22825	4648972..4649229	+	258	WP_193333056.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (91-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 179 a.a.
• Molecular weight: 19969.90 Da
• Isoelectric Point: 5.2614

>T158899 WP_193333055.1 NZ_CP054170:c4644835-4644299 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSN
LLFYPTKSIEQLFTDDES

Nucleotide

• Length: 537 bp

>T158899 NZ_CP069882:c3295826-3295719 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10095.59 Da
• Isoelectric Point: 10.1757

>AT158899 WP_151361316.1 NZ_CP054170:c4645110-4644841 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTVR

Nucleotide

• Length: 270 bp

>AT158899 NZ_CP069882:3295874-3295940 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTATACCTCTCAACGTGCGGGGGTTTTCTC