Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4013304..4013890 Replicon chromosome
Accession NZ_CP054169
Organism Escherichia coli strain STO_Bone1B

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag HRS04_RS20440 Protein ID WP_042014162.1
Coordinates 4013660..4013890 (-) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag HRS04_RS20435 Protein ID WP_000593555.1
Coordinates 4013304..4013663 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRS04_RS20410 (4009436) 4009436..4010380 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
HRS04_RS20415 (4010377) 4010377..4011210 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
HRS04_RS20420 (4011210) 4011210..4011974 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
HRS04_RS20425 (4011971) 4011971..4012777 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
HRS04_RS20430 (4012783) 4012783..4013184 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
HRS04_RS20435 (4013304) 4013304..4013663 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HRS04_RS20440 (4013660) 4013660..4013890 - 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
HRS04_RS20445 (4013994) 4013994..4015118 - 1125 WP_001314210.1 ABC transporter permease -
HRS04_RS20450 (4015118) 4015118..4017853 - 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T158881 WP_042014162.1 NZ_CP054169:c4013890-4013660 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T158881 NZ_CP069873:2320115-2320648 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT158881 WP_000593555.1 NZ_CP054169:c4013663-4013304 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT158881 NZ_CP069873:2319838-2320104 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References