Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3258364..3258947 Replicon chromosome
Accession NZ_CP054169
Organism Escherichia coli strain STO_Bone1B

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag HRS04_RS16760 Protein ID WP_000254745.1
Coordinates 3258364..3258699 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A3P5DUY8
Locus tag HRS04_RS16765 Protein ID WP_074502498.1
Coordinates 3258699..3258947 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRS04_RS16740 (3253833) 3253833..3254195 + 363 WP_000034929.1 hypothetical protein -
HRS04_RS16745 (3254251) 3254251..3255549 - 1299 WP_000036723.1 phosphopyruvate hydratase -
HRS04_RS16750 (3255637) 3255637..3257274 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
HRS04_RS16755 (3257502) 3257502..3258293 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
HRS04_RS16760 (3258364) 3258364..3258699 - 336 WP_000254745.1 endoribonuclease MazF Toxin
HRS04_RS16765 (3258699) 3258699..3258947 - 249 WP_074502498.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
HRS04_RS16770 (3259025) 3259025..3261259 - 2235 WP_000226815.1 GTP pyrophosphokinase -
HRS04_RS16775 (3261307) 3261307..3262608 - 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T158877 WP_000254745.1 NZ_CP054169:c3258699-3258364 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T158877 NZ_CP069873:1712443-1712826 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9359.72 Da        Isoelectric Point: 4.4791

>AT158877 WP_074502498.1 NZ_CP054169:c3258947-3258699 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNEITPENLHENIDWGESKDKE
VW

Download         Length: 249 bp

>AT158877 NZ_CP069873:1712201-1712443 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
AlphaFold DB A0A3P5DUY8

References