Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 907669..908287 Replicon chromosome
Accession NZ_CP054169
Organism Escherichia coli strain STO_Bone1B

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HRS04_RS05340 Protein ID WP_001291435.1
Coordinates 907669..907887 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HRS04_RS05345 Protein ID WP_000344800.1
Coordinates 907913..908287 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRS04_RS05305 (902959) 902959..903531 + 573 WP_000779826.1 YbaY family lipoprotein -
HRS04_RS05310 (903562) 903562..903873 - 312 WP_000409911.1 MGMT family protein -
HRS04_RS05320 (904252) 904252..904605 + 354 WP_000878140.1 DUF1428 domain-containing protein -
HRS04_RS05325 (904647) 904647..906197 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HRS04_RS05330 (906361) 906361..906831 - 471 WP_000136192.1 YlaC family protein -
HRS04_RS05335 (906947) 906947..907498 - 552 WP_000102564.1 maltose O-acetyltransferase -
HRS04_RS05340 (907669) 907669..907887 - 219 WP_001291435.1 HHA domain-containing protein Toxin
HRS04_RS05345 (907913) 907913..908287 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HRS04_RS05350 (908833) 908833..911982 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
HRS04_RS05355 (912005) 912005..913198 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T158858 WP_001291435.1 NZ_CP054169:c907887-907669 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T158858 NZ_CP069865:2363487-2363798 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT158858 WP_000344800.1 NZ_CP054169:c908287-907913 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT158858 NZ_CP069865:2363799-2364089 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References