158857

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 873373..874210 Replicon chromosome
Accession NZ_CP054169
Organism Escherichia coli strain STO_Bone1B

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag HRS04_RS05170 Protein ID WP_000227784.1
Coordinates 873373..873915 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag HRS04_RS05175 Protein ID WP_001297137.1
Coordinates 873899..874210 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRS04_RS05150 (868912) 868912..869823 - 912 WP_000705874.1 2-dehydropantoate 2-reductase -
HRS04_RS05155 (869991) 869991..870482 + 492 WP_001138904.1 nucleotide binding protein YajQ -
HRS04_RS05160 (870610) 870610..871974 - 1365 WP_001000978.1 MFS transporter -
HRS04_RS05165 (872382) 872382..873317 + 936 WP_001297127.1 tetratricopeptide repeat protein -
HRS04_RS05170 (873373) 873373..873915 - 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
HRS04_RS05175 (873899) 873899..874210 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
HRS04_RS05180 (874395) 874395..875285 - 891 WP_000971336.1 heme o synthase -
HRS04_RS05185 (875297) 875297..875626 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
HRS04_RS05190 (875626) 875626..876240 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
HRS04_RS05195 (876230) 876230..878221 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
HRS04_RS05200 (878243) 878243..879190 - 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T158857 WP_000227784.1 NZ_CP054169:c873915-873373 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T158857 NZ_CP069865:c1940778-1940671 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGCATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT158857 WP_001297137.1 NZ_CP054169:c874210-873899 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT158857 NZ_CP069865:1940836-1940890 [Escherichia coli]
CAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References