Detailed information of TA system TA158853

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 244987..245819
• Replicon: chromosome
• Accession: NZ_CP054169
• Organism: Escherichia coli strain STO_Bone1B

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A7ZVJ9
• Locus tag: HRS04_RS02285
• Protein ID: WP_000854765.1
• Coordinates: 245445..245819 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: HRS04_RS02280
• Protein ID: WP_001295723.1
• Coordinates: 244987..245355 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRS04_RS02250 (241823)	241823..242278	+	456	WP_000581504.1	IrmA family protein	-
HRS04_RS02255 (242357)	242357..242590	+	234	WP_001119729.1	DUF905 family protein	-
HRS04_RS02260 (242690)	242690..243508	+	819	WP_001234620.1	DUF932 domain-containing protein	-
HRS04_RS02265 (243563)	243563..244048	+	486	WP_000849588.1	antirestriction protein	-
HRS04_RS02270 (244064)	244064..244540	+	477	WP_001186774.1	RadC family protein	-
HRS04_RS02275 (244603)	244603..244824	+	222	WP_000692311.1	DUF987 domain-containing protein	-
HRS04_RS02280 (244987)	244987..245355	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HRS04_RS02285 (245445)	245445..245819	+	375	WP_000854765.1	TA system toxin CbtA family protein	Toxin
HRS04_RS02290 (245816)	245816..246307	+	492	WP_000976842.1	DUF5983 family protein	-
HRS04_RS02295 (246324)	246324..246500	+	177	WP_000839290.1	DUF957 domain-containing protein	-
HRS04_RS02300 (246606)	246606..246755	+	150	Protein_250	hypothetical protein	-
HRS04_RS02310 (247886)	247886..248056	+	171	Protein_252	fimbrial protein	-
HRS04_RS02315 (248230)	248230..249573	-	1344	WP_000558251.1	gluconate permease GntP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	247134..247637	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13970.98 Da
• Isoelectric Point: 7.2909

>T158853 WP_000854765.1 NZ_CP054169:245445-245819 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T158853 NZ_CP069865:c1427242-1426946 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT158853 WP_001295723.1 NZ_CP054169:244987-245355 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT158853 NZ_CP069865:c1426944-1426549 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• AlphaFold DB: A7ZVJ9

Antitoxin

• AlphaFold DB: S1NM52