Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 147740..148341 Replicon plasmid pBone1BIncF
Accession NZ_CP054166
Organism Escherichia coli strain STO_Bone1B

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag HRS04_RS00835 Protein ID WP_001216034.1
Coordinates 147961..148341 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag HRS04_RS00830 Protein ID WP_001190712.1
Coordinates 147740..147961 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRS04_RS00820 (144721) 144721..146004 - 1284 WP_001553855.1 restriction endonuclease subunit S -
HRS04_RS00825 (146001) 146001..147557 - 1557 WP_001553856.1 type I restriction-modification system subunit M -
HRS04_RS00830 (147740) 147740..147961 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
HRS04_RS00835 (147961) 147961..148341 + 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
HRS04_RS00840 (148346) 148346..148525 + 180 WP_001513661.1 hypothetical protein -
HRS04_RS00845 (148553) 148553..148831 + 279 Protein_168 pdcB -
HRS04_RS00850 (148836) 148836..149249 + 414 Protein_169 integrase core domain-containing protein -
HRS04_RS00855 (149199) 149199..149534 - 336 WP_169329198.1 type I deoxyribonuclease HsdR -
HRS04_RS00860 (149744) 149744..150724 - 981 WP_000019407.1 IS5-like element IS5 family transposase -
HRS04_RS00865 (150968) 150968..152371 + 1404 WP_001373486.1 S-methylmethionine permease -
HRS04_RS00870 (152358) 152358..153290 + 933 WP_000081352.1 homocysteine S-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(3)-IIa / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / tet(A) - 1..165243 165243


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T158852 WP_001216034.1 NZ_CP054166:147961-148341 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T158852 NZ_CP069865:c1308291-1307884 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATCTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGAGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT158852 WP_001190712.1 NZ_CP054166:147740-147961 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT158852 NZ_CP069865:c1308538-1308272 [Escherichia coli]
ATGGACATTAATAACAAAGCCCGCATTCACTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGAATCTTTATTCGTCTGCTGGAGTGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAGCTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References