Detailed information of TA system TA158844

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 62498..63141
• Replicon: plasmid pBone2IncF
• Accession: NZ_CP054165
• Organism: Escherichia coli strain STO_Bone2

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HRS05_RS25580
• Protein ID: WP_016236302.1
• Coordinates: 62725..63141 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: HRS05_RS25575
• Protein ID: WP_001261282.1
• Coordinates: 62498..62728 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRS05_RS25555 (59047)	59047..59802	-	756	WP_000852146.1	replication initiation protein RepE	-
HRS05_RS25560 (60576)	60576..61382	-	807	WP_000016982.1	site-specific integrase	-
HRS05_RS25565 (61383)	61383..61688	-	306	WP_001159868.1	type II toxin-antitoxin system toxin CcdB	-
HRS05_RS25570 (61690)	61690..61908	-	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	-
HRS05_RS25575 (62498)	62498..62728	+	231	WP_001261282.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HRS05_RS25580 (62725)	62725..63141	+	417	WP_016236302.1	type II toxin-antitoxin system VapC family toxin	Toxin
HRS05_RS25585 (63215)	63215..64777	+	1563	WP_004206609.1	AAA family ATPase	-
HRS05_RS25590 (64762)	64762..65784	+	1023	WP_000361404.1	helicase UvrD	-
HRS05_RS25595 (65965)	65965..66195	-	231	Protein_78	IS1 family transposase	-
HRS05_RS25600 (66250)	66250..66954	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
HRS05_RS25605 (67533)	67533..67880	+	348	WP_000609174.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	catB3 / blaOXA-1 / aac(6')-Ib-cr / aadA5 / qacE / sul1	-	1..68411	68411

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15091.55 Da
• Isoelectric Point: 7.1084

>T158844 WP_016236302.1 NZ_CP054165:62725-63141 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T158844 NZ_CP069863:c183490-183206 [Klebsiella pneumoniae]
ATGACCTATAAGCTGGCTTTCAACGAATCCGCTCTGAAAGAATGGAAGAAGCTGGGTCATACACTACAGGTACAGTTCAA
AAAGAAACTCAAAGAGCGCCTGGAGAACCCCAGAGTTCCGGCATCACAGCTACATGGACGTAAGGACCAGTATAAAATTA
AGCTACGTGGTGCCGGATATCGCCTTGTCTATCGTGTAGAGGACGACATTGTCACAGTCACTGTTATCGGCGTAGGCAAA
CGCGAAAACGACGACATTTATAATGCAACCCTTAATCGCAATTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT158844 WP_001261282.1 NZ_CP054165:62498-62728 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT158844 NZ_CP069863:c183728-183480 [Klebsiella pneumoniae]
ATGGCATTTCAGGTTCTGACCACTACAGCAGCTAGCATTACTGAACTTAAACGTGACCCTATGGGTACTTTCCACGCCGG
GGATGGCGCTCCGGTAGCCATTCTTAATCGTAATGAGCCTGCATTCTACTGTGTGCCACCGGCACTCTATGCCCAGTTGA
TGGAGCTTCTGGAAGATGAAGAGCTGGGACGGATGGCTGATGAGCGAAGCGGAGAAAAGGTTATAGAGGTCAATATTGAT
GACCTATAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3