Detailed information of TA system TA158843

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 61383..61908
• Replicon: plasmid pBone2IncF
• Accession: NZ_CP054165
• Organism: Escherichia coli strain STO_Bone2

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: V0SSI5
• Locus tag: HRS05_RS25565
• Protein ID: WP_001159868.1
• Coordinates: 61383..61688 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1PPD8
• Locus tag: HRS05_RS25570
• Protein ID: WP_000813634.1
• Coordinates: 61690..61908 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRS05_RS25550 (57293)	57293..58459	-	1167	WP_000772446.1	plasmid-partitioning protein SopA	-
HRS05_RS25555 (59047)	59047..59802	-	756	WP_000852146.1	replication initiation protein RepE	-
HRS05_RS25560 (60576)	60576..61382	-	807	WP_000016982.1	site-specific integrase	-
HRS05_RS25565 (61383)	61383..61688	-	306	WP_001159868.1	type II toxin-antitoxin system toxin CcdB	Toxin
HRS05_RS25570 (61690)	61690..61908	-	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
HRS05_RS25575 (62498)	62498..62728	+	231	WP_001261282.1	type II toxin-antitoxin system VapB family antitoxin	-
HRS05_RS25580 (62725)	62725..63141	+	417	WP_016236302.1	type II toxin-antitoxin system VapC family toxin	-
HRS05_RS25585 (63215)	63215..64777	+	1563	WP_004206609.1	AAA family ATPase	-
HRS05_RS25590 (64762)	64762..65784	+	1023	WP_000361404.1	helicase UvrD	-
HRS05_RS25595 (65965)	65965..66195	-	231	Protein_78	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	catB3 / blaOXA-1 / aac(6')-Ib-cr / aadA5 / qacE / sul1	-	1..68411	68411

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11706.51 Da
• Isoelectric Point: 6.4674

>T158843 WP_001159868.1 NZ_CP054165:c61688-61383 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T158843 NZ_CP069863:180205-180675 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGAATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAATTGATCCAAAGCGCAAATATGGAT
GAATTACCGGCCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCTCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACTCTGATTTTT
ACGGGATCGTGCAGATGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.34 Da
• Isoelectric Point: 4.7405

>AT158843 WP_000813634.1 NZ_CP054165:c61908-61690 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT158843 NZ_CP069863:179765-180208 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATAG
CGTAGCGCTGCTGGGTCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAACCGGATCACTGACTGGGCCAGAATAACCC
AAAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGACTGGTACGTATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGATAAGGAAGCCGCACTGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATAGCATCCGAGACGGGAGCATATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• PDB: 4VUB
• PDB: 2VUB
• PDB: 7EPI
• PDB: 7EPG
• PDB: 3VUB
• PDB: 1X75
• PDB: 1VUB
• PDB: 7EPJ
• PDB: 3HPW
• PDB: 3G7Z

Antitoxin

• PDB: 3TCJ
• PDB: 2H3A
• PDB: 2ADN
• PDB: 2ADL
• PDB: 3HPW
• PDB: 2H3C
• PDB: 3G7Z
• AlphaFold DB: A0A829CQY2