Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4872519..4873137 Replicon chromosome
Accession NZ_CP054161
Organism Escherichia coli strain STO_Bone2

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HRS05_RS23400 Protein ID WP_001291435.1
Coordinates 4872919..4873137 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HRS05_RS23395 Protein ID WP_000344800.1
Coordinates 4872519..4872893 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRS05_RS23385 (4867608) 4867608..4868801 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HRS05_RS23390 (4868824) 4868824..4871973 + 3150 WP_235163822.1 efflux RND transporter permease AcrB -
HRS05_RS23395 (4872519) 4872519..4872893 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HRS05_RS23400 (4872919) 4872919..4873137 + 219 WP_001291435.1 HHA domain-containing protein Toxin
HRS05_RS23405 (4873309) 4873309..4873860 + 552 WP_001522034.1 maltose O-acetyltransferase -
HRS05_RS23410 (4873976) 4873976..4874446 + 471 WP_001490888.1 YlaC family protein -
HRS05_RS23415 (4874610) 4874610..4876160 + 1551 WP_001577872.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HRS05_RS23420 (4876202) 4876202..4876555 - 354 WP_000878140.1 DUF1428 domain-containing protein -
HRS05_RS23430 (4876934) 4876934..4877245 + 312 WP_000409911.1 MGMT family protein -
HRS05_RS23435 (4877276) 4877276..4877848 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T158838 WP_001291435.1 NZ_CP054161:4872919-4873137 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T158838 NZ_CP069861:1709979-1710263 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT158838 WP_000344800.1 NZ_CP054161:4872519-4872893 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT158838 NZ_CP069861:1709747-1709989 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References