Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4313939..4314734 Replicon chromosome
Accession NZ_CP054161
Organism Escherichia coli strain STO_Bone2

Toxin (Protein)


Gene name yeeV Uniprot ID B7UP43
Locus tag HRS05_RS20885 Protein ID WP_000854914.1
Coordinates 4313939..4314313 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B7UP42
Locus tag HRS05_RS20890 Protein ID WP_001280955.1
Coordinates 4314360..4314734 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRS05_RS20855 (4310617) 4310617..4311321 + 705 WP_001241678.1 leucyl/phenylalanyl-tRNA--protein transferase -
HRS05_RS20860 (4311606) 4311606..4311824 + 219 WP_001040187.1 translation initiation factor IF-1 -
HRS05_RS20870 (4312315) 4312315..4313157 - 843 WP_001280481.1 DUF4942 domain-containing protein -
HRS05_RS20875 (4313242) 4313242..4313439 - 198 WP_000839282.1 DUF957 domain-containing protein -
HRS05_RS20880 (4313451) 4313451..4313942 - 492 WP_000976857.1 DUF5983 family protein -
HRS05_RS20885 (4313939) 4313939..4314313 - 375 WP_000854914.1 TA system toxin CbtA family protein Toxin
HRS05_RS20890 (4314360) 4314360..4314734 - 375 WP_001280955.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HRS05_RS20895 (4314897) 4314897..4315118 - 222 WP_001220314.1 DUF987 domain-containing protein -
HRS05_RS20900 (4315181) 4315181..4315657 - 477 WP_001186738.1 RadC family protein -
HRS05_RS20905 (4315673) 4315673..4316158 - 486 WP_000214398.1 antirestriction protein -
HRS05_RS20910 (4316249) 4316249..4317067 - 819 WP_001234682.1 DUF932 domain-containing protein -
HRS05_RS20915 (4317407) 4317407..4318477 - 1071 WP_000102669.1 patatin-like phospholipase family protein -
HRS05_RS20920 (4318474) 4318474..4319379 - 906 WP_023149949.1 diguanylate cyclase regulator RdcB family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14117.17 Da        Isoelectric Point: 7.7761

>T158836 WP_000854914.1 NZ_CP054161:c4314313-4313939 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T158836 NZ_CP069861:1230666-1231049 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGTACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13767.59 Da        Isoelectric Point: 6.6248

>AT158836 WP_001280955.1 NZ_CP054161:c4314734-4314360 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Download         Length: 375 bp

>AT158836 NZ_CP069861:1230424-1230666 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LXR5

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9P0D0

References