Detailed information of TA system TA158832

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3601177..3601815
• Replicon: chromosome
• Accession: NZ_CP054161
• Organism: Escherichia coli strain STO_Bone2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0D8WF76
• Locus tag: HRS05_RS17285
• Protein ID: WP_001306887.1
• Coordinates: 3601639..3601815 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HRS05_RS17280
• Protein ID: WP_001270286.1
• Coordinates: 3601177..3601593 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRS05_RS17260 (3596329)	3596329..3597270	-	942	WP_001251315.1	ABC transporter permease	-
HRS05_RS17265 (3597271)	3597271..3598284	-	1014	WP_001523231.1	ABC transporter ATP-binding protein	-
HRS05_RS17270 (3598302)	3598302..3599447	-	1146	WP_020233879.1	ABC transporter substrate-binding protein	-
HRS05_RS17275 (3599692)	3599692..3601098	-	1407	WP_001523229.1	PLP-dependent aminotransferase family protein	-
HRS05_RS17280 (3601177)	3601177..3601593	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HRS05_RS17285 (3601639)	3601639..3601815	-	177	WP_001306887.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HRS05_RS17290 (3602037)	3602037..3602267	+	231	WP_000494241.1	YncJ family protein	-
HRS05_RS17295 (3602359)	3602359..3604320	-	1962	WP_057950830.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HRS05_RS17300 (3604393)	3604393..3604929	-	537	WP_020233877.1	DNA-binding transcriptional regulator SutR	-
HRS05_RS17305 (3605021)	3605021..3606193	+	1173	WP_001523225.1	BenE family transporter YdcO	-
HRS05_RS17310 (3606301)	3606301..3606759	-	459	WP_000526135.1	IS200/IS605-like element IS200C family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3606301..3606759	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6768.88 Da
• Isoelectric Point: 11.5336

>T158832 WP_001306887.1 NZ_CP054161:c3601815-3601639 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFRGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T158832 NZ_CP069861:c329447-329058 [Klebsiella pneumoniae]
ATGCAAACCAAATCACCCCCCTTCATGCGGGCGGCATCGTCACGTCCGTCTCCCGTGGGCGTCTGGCAGACCCTGCTGAC
CTGCCTGCTGGAACACCACTATGGCCTTACGCTTAACGATACGCCGTTCAGTGATGAACTGGTGATTCAGCAACATATCG
AGGCGGGTATCTCGCTGGCCGATGCCCTGAATTTTATTGTTGAAAAGTATGAACTGGTCCGCACTGACCGGCCAGGCTTC
AGCATCAGGGAGCAGTCTCCGTTTATTACATCCATTGATATCCTCCGGGCCAGAAAGGCAACGGGGCTGATGAATTGTGG
CACGTATAAAGATGTGACGGCCATTACCAGAGGACTGCATAGACAGGTCAGCACCCCCGGTAAACGCTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT158832 WP_001270286.1 NZ_CP054161:c3601593-3601177 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT158832 NZ_CP069861:c329862-329503 [Klebsiella pneumoniae]
TTGTCGAAGAAAACCACGATAGCAACGCATGATATCAGCGAATCCTGGTGGGGGCTCAGACGCAGTGTCTCCGCGTGTTT
TGGTGCCCGACTGGTCCAGGAGGGAAACCGCCTGCATTACCTGGCTGACCGCGCGAATTTTAACGGACAGTTCTGCGATG
CGGATTTGCGCCATCTTGACCAGTCTTTCCCGGTATTGATGAAACAGCTGGAGTTAATGCTCACCAGCGGTGAACTGAAT
CCCCGTCATCAGCACTGCGTCACGCTGTATGCAAAAGGTCTGACCTGCGAGGCCGATACGCTCGGTTCGCATGGCTACGT
TTATATCGCGATTTATCCCACTCCCGTCACCACAGAATAA

Structures

Toxin

• AlphaFold DB: A0A0D8WF76