Detailed information of TA system TA158813

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 360040..360635
• Replicon: chromosome
• Accession: NZ_CP054161
• Organism: Escherichia coli strain STO_Bone2

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: HRS05_RS01740
• Protein ID: WP_000239581.1
• Coordinates: 360040..360390 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: HRS05_RS01745
• Protein ID: WP_001223213.1
• Coordinates: 360384..360635 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRS05_RS01720 (355494)	355494..356516	-	1023	WP_001361374.1	ABC transporter permease	-
HRS05_RS01725 (356530)	356530..358032	-	1503	WP_001522399.1	sugar ABC transporter ATP-binding protein	-
HRS05_RS01730 (358165)	358165..359121	-	957	WP_001522398.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
HRS05_RS01735 (359431)	359431..359961	+	531	WP_000055075.1	inorganic diphosphatase	-
HRS05_RS01740 (360040)	360040..360390	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
HRS05_RS01745 (360384)	360384..360635	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
HRS05_RS01750 (360847)	360847..361188	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
HRS05_RS01755 (361191)	361191..364970	-	3780	WP_001522396.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T158813 WP_000239581.1 NZ_CP054161:c360390-360040 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T158813 NZ_CP069847:3717535-3717903 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGCGGATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT158813 WP_001223213.1 NZ_CP054161:c360635-360384 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT158813 NZ_CP069847:3717317-3717538 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7