Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4960237..4960790 Replicon chromosome
Accession NZ_CP054160
Organism Serratia fonticola strain HH13

Toxin (Protein)


Gene name ccdB Uniprot ID U2NFJ3
Locus tag G9399_RS22575 Protein ID WP_021179490.1
Coordinates 4960476..4960790 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1C7WDQ0
Locus tag G9399_RS22570 Protein ID WP_065684692.1
Coordinates 4960237..4960473 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9399_RS22555 (G9399_03820) 4956332..4957471 - 1140 WP_065684689.1 FMN-dependent L-lactate dehydrogenase LldD -
G9399_RS22560 (G9399_03815) 4957488..4958270 - 783 WP_065684690.1 transcriptional regulator LldR -
G9399_RS22565 (G9399_03810) 4958267..4959922 - 1656 WP_098929751.1 L-lactate permease -
G9399_RS22570 (G9399_03805) 4960237..4960473 + 237 WP_065684692.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
G9399_RS22575 (G9399_03800) 4960476..4960790 + 315 WP_021179490.1 CcdB family protein Toxin
G9399_RS22580 (G9399_03795) 4960896..4961723 - 828 WP_065684693.1 undecaprenyl-diphosphate phosphatase -
G9399_RS22585 (G9399_03790) 4961864..4962223 - 360 WP_024483059.1 bifunctional dihydroneopterin aldolase/7,8-dihydroneopterin epimerase -
G9399_RS22590 (G9399_03785) 4962331..4962969 + 639 WP_024528268.1 glycerol-3-phosphate 1-O-acyltransferase PlsY -
G9399_RS22595 (G9399_03780) 4963178..4964110 - 933 WP_173408707.1 winged helix-turn-helix domain-containing protein -
G9399_RS22600 (G9399_03775) 4964835..4965215 + 381 WP_173408706.1 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(9-78)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11579.56 Da        Isoelectric Point: 6.9725

>T158810 WP_021179490.1 NZ_CP054160:4960476-4960790 [Serratia fonticola]
MQFTVYYNPGKSVVYPLLLDVTSDIIGQLNRRIVIPLLPVEKYPGSSRPDRMNPVIMLTDGKEYAVMAHELASIPVRALG
AEFCNAAQYRPQIKAAIDFLIDGI

Download         Length: 315 bp

>T158810 NZ_CP069847:c2841875-2841591 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCTGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8870.92 Da        Isoelectric Point: 7.3600

>AT158810 WP_065684692.1 NZ_CP054160:4960237-4960473 [Serratia fonticola]
MTVKVRNTQSVTMTVERGLLNRARDAGINLSATLTAALDAELRHHAAKKWQEENRGALEALNRFHDEHGCFSDEYRTF

Download         Length: 237 bp

>AT158810 NZ_CP069847:c2842107-2841865 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U2NFJ3

Antitoxin

Source ID Structure
AlphaFold DB A0A1C7WDQ0

References