Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF-MazE
Location 796383..797008 Replicon chromosome
Accession NZ_CP054150
Organism Agrobacterium vaccinii strain B7.6

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag HRR99_RS04030 Protein ID WP_233122866.1
Coordinates 796649..797008 (+) Length 120 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A081CY97
Locus tag HRR99_RS04025 Protein ID WP_045231190.1
Coordinates 796383..796649 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRR99_RS04005 (HRR99_03990) 792081..792827 - 747 WP_233122863.1 hypothetical protein -
HRR99_RS04010 (HRR99_03995) 792971..795634 + 2664 WP_277877898.1 heavy metal translocating P-type ATPase -
HRR99_RS04015 (HRR99_04000) 795631..796041 + 411 WP_233122864.1 Cu(I)-responsive transcriptional regulator -
HRR99_RS04020 (HRR99_04005) 796068..796268 + 201 WP_233122865.1 heavy-metal-associated domain-containing protein -
HRR99_RS04025 (HRR99_04010) 796383..796649 + 267 WP_045231190.1 PbsX family transcriptional regulator Antitoxin
HRR99_RS04030 (HRR99_04015) 796649..797008 + 360 WP_233122866.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HRR99_RS04035 (HRR99_04020) 797017..797205 + 189 WP_233122867.1 hypothetical protein -
HRR99_RS04040 (HRR99_04025) 797221..797469 - 249 WP_233122868.1 hypothetical protein -
HRR99_RS04045 (HRR99_04030) 797603..799024 + 1422 WP_233122869.1 MFS transporter -
HRR99_RS04050 (HRR99_04035) 799019..799189 - 171 WP_164470675.1 hypothetical protein -
HRR99_RS04055 (HRR99_04040) 799256..800971 - 1716 WP_233122870.1 GH32 C-terminal domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 120 a.a.        Molecular weight: 12800.08 Da        Isoelectric Point: 8.7910

>T158788 WP_233122866.1 NZ_CP054150:796649-797008 [Agrobacterium vaccinii]
MVRSNVPKRGDVFLIDLNPVVGNEMKDEHRCVVITPKEINAVGLCLVVPITTGGMFTRKAGLAVNISGQRTTGVALCNQV
RSLDIAGRIALKKAKFLETLDDATMQEIVARVVSVIDPA

Download         Length: 360 bp

>T158788 NZ_CP069821:c3935647-3935165 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9371.82 Da        Isoelectric Point: 4.5510

>AT158788 WP_045231190.1 NZ_CP054150:796383-796649 [Agrobacterium vaccinii]
MTVTTKIRRQGGAAVMTIPPALLKLMGTEIGSEMTLEIDNGNLVASPVTTQKKRYSLAELLEGADEMAELNVEASAWDAA
PRVGKEAL

Download         Length: 267 bp

>AT158788 NZ_CP069821:c3935901-3935635 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A081CY97

References