Detailed information of TA system TA158784

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 64254..64852
• Replicon: plasmid pB19.1.4a
• Accession: NZ_CP054148
• Organism: Agrobacterium vaccinii strain B19.1.4

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HRS00_RS22785
• Protein ID: WP_233137960.1
• Coordinates: 64559..64852 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HRS00_RS22780
• Protein ID: WP_233137959.1
• Coordinates: 64254..64562 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRS00_RS22765 (HRS00_22705)	60396..61025	+	630	WP_233137956.1	hypothetical protein	-
HRS00_RS22770 (HRS00_22710)	61279..63594	-	2316	WP_233137957.1	hypothetical protein	-
HRS00_RS22775 (HRS00_22715)	63781..64182	+	402	WP_233137958.1	DUF2513 domain-containing protein	-
HRS00_RS22780 (HRS00_22720)	64254..64562	+	309	WP_233137959.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
HRS00_RS22785 (HRS00_22725)	64559..64852	+	294	WP_233137960.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HRS00_RS22790 (HRS00_22730)	65065..65871	+	807	WP_233137961.1	GGDEF domain-containing protein	-
HRS00_RS22795 (HRS00_22735)	66290..66544	-	255	WP_233137962.1	hypothetical protein	-
HRS00_RS22800 (HRS00_22740)	67495..69034	+	1540	Protein_67	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..218909	218909

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-56)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10703.35 Da
• Isoelectric Point: 5.8086

>T158784 WP_233137960.1 NZ_CP054148:64559-64852 [Agrobacterium vaccinii]
MRLTWSAFALSDRDAIFTFIELENPAAAILVDERIVVAVRRLIDFPASGRVGRIAGTRELVISGTPYVAAYTITEATVRV
LRVLHGAQEWPEAFSIS

Nucleotide

• Length: 294 bp

>T158784 NZ_CP069821:829619-830152 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11162.48 Da
• Isoelectric Point: 4.6787

>AT158784 WP_233137959.1 NZ_CP054148:64254-64562 [Agrobacterium vaccinii]
MAANALVQTRIDADVRDRASLVLEGMGLTVSDAVRILLTRTANEGVLPLELFSGSEAHDTWFRAKVLEALNDTRPDISDD
EVEAHFEKRRQAARNNAAGITS

Nucleotide

• Length: 309 bp

>AT158784 NZ_CP069821:829342-829608 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA